The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Titre using peptide based assay: 1/62500.
Use a concentration of 1.25 µg/ml. Predicted molecular weight: 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
FunctionSubstrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT).
Tissue specificityExpressed in all brain tissue observed.
PathwayProtein modification; protein ubiquitination.
Involvement in diseaseNote=A chromosomal aberration involving FBXO25 is a cause of X-linked mental retardation (XLMR). Translocation t(X;8)(p11.22;p23.3) with SHROOM4.
Sequence similaritiesContains 1 F-box domain.
DomainThe F-box is necessary for the interaction with SKP1.
Cellular localizationNucleus. In the nucleus, associates with a subnuclear dot-like structure. Colocalized with SKP1.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling FBXO25 with ab58784 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of renal tubule. Magnification: 400X.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using ab58784 at a dilution of 4-8 µg/ml, positively labelling epithelial cells of renal tubule (indicated with arrows). Magnification 400X
References for Anti-FBXO25 antibody (ab58784)
has not yet been referenced specifically in any publications.
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