Overview

  • Product nameAnti-FBXO25 antibody
    See all FBXO25 primary antibodies
  • Description
    Rabbit polyclonal to FBXO25
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: EQYGDTLHFC RHCSILFWKD SGHPCTAADP DSCFTPVSPQ HFIDLFKF, corresponding to C terminal amino acids 244-291 of Human FBXO25

  • Positive control
    • HepG2 cell lysate; human kidney tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab58784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionSubstrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT).
  • Tissue specificityExpressed in all brain tissue observed.
  • PathwayProtein modification; protein ubiquitination.
  • Involvement in diseaseNote=A chromosomal aberration involving FBXO25 is a cause of X-linked mental retardation (XLMR). Translocation t(X;8)(p11.22;p23.3) with SHROOM4.
  • Sequence similaritiesContains 1 F-box domain.
  • DomainThe F-box is necessary for the interaction with SKP1.
  • Cellular localizationNucleus. In the nucleus, associates with a subnuclear dot-like structure. Colocalized with SKP1.
  • Information by UniProt
  • Database links
  • Alternative names
    • F box only protein 25 antibody
    • F box protein 25 antibody
    • F box protein Fbx25 antibody
    • F-box only protein 25 antibody
    • FBX25 antibody
    • FBX25_HUMAN antibody
    • FBXO25 antibody
    • MGC20256 antibody
    • MGC51975 antibody
    • OTTHUMP00000115399 antibody
    see all

Anti-FBXO25 antibody images

  • Anti-FBXO25 antibody (ab58784) at 1.25 µg/ml + HepG2 cell lysate at 10 µg with 5% skim milk in PBS buffer

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 43 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    Gel concentration 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling FBXO25 with ab58784 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of renal tubule. Magnification: 400X.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using ab58784 at a dilution of 4-8 µg/ml, positively labelling epithelial cells of renal tubule (indicated with arrows). Magnification 400X

References for Anti-FBXO25 antibody (ab58784)

ab58784 has not yet been referenced specifically in any publications.

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