The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay, 1/312500.
FunctionSubstrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT).
Tissue specificityExpressed in all brain tissue observed.
PathwayProtein modification; protein ubiquitination.
Involvement in diseaseNote=A chromosomal aberration involving FBXO25 is a cause of X-linked mental retardation (XLMR). Translocation t(X;8)(p11.22;p23.3) with SHROOM4.
Sequence similaritiesContains 1 F-box domain.
DomainThe F-box is necessary for the interaction with SKP1.
Cellular localizationNucleus. In the nucleus, associates with a subnuclear dot-like structure. Colocalized with SKP1.