Overview

  • Product name
    Anti-FBXO25 antibody
  • Description
    Rabbit polyclonal to FBXO25
  • Specificity
    ab82971 detects isoform 3.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 36 - 85 (LGEAFNRLDF SSAIQDIRRF NYVVKLLQLI AKSQLTSLSG VAQKNYFNIL) of human FBXO25 (NP_036305).

  • Positive control
    • Fetal heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab82971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1/312500.

Target

  • Function
    Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT).
  • Tissue specificity
    Expressed in all brain tissue observed.
  • Pathway
    Protein modification; protein ubiquitination.
  • Involvement in disease
    Note=A chromosomal aberration involving FBXO25 is a cause of X-linked mental retardation (XLMR). Translocation t(X;8)(p11.22;p23.3) with SHROOM4.
  • Sequence similarities
    Contains 1 F-box domain.
  • Domain
    The F-box is necessary for the interaction with SKP1.
  • Cellular localization
    Nucleus. In the nucleus, associates with a subnuclear dot-like structure. Colocalized with SKP1.
  • Information by UniProt
  • Database links
  • Alternative names
    • F box only protein 25 antibody
    • F box protein 25 antibody
    • F box protein Fbx25 antibody
    • F-box only protein 25 antibody
    • FBX25 antibody
    • FBX25_HUMAN antibody
    • FBXO25 antibody
    • MGC20256 antibody
    • MGC51975 antibody
    • OTTHUMP00000115399 antibody
    see all

Images



  • Predicted band size : 34 kDa
    Western bloat analysis of human brain tissue lysate labelling FBXO25 with ab82971 at 0.2-1µg/ml.
  • Anti-FBXO25 antibody (ab82971) at 1 µg/ml + fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 34 kDa
    Observed band size : 34 kDa

References

ab82971 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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