Anti-FBXO30 antibody (ab68224)
Key features and details
- Mouse polyclonal to FBXO30
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FBXO30 antibody
See all FBXO30 primary antibodies -
Description
Mouse polyclonal to FBXO30 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human FBXO30. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- FBXO30 transfected 293T cell lysate and human colon tissue lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituents: 8% Sodium chloride, 0.2% Potassium chloride, 0.2% Monobasic dihydrogen potassium phosphate, 0.6% Dibasic monohydrogen sodium phosphate, 91% Water -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 37, 82 kDa (predicted molecular weight: 82 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 37, 82 kDa (predicted molecular weight: 82 kDa). |
Target
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Relevance
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and it is upregulated in nasopharyngeal carcinoma. [Entrez Gene] -
Database links
- Entrez Gene: 84085 Human
- Omim: 609101 Human
- SwissProt: Q8TB52 Human
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Alternative names
- F box domain protein antibody
- F box only protein 30 antibody
- F box only protein, helicase, 18 antibody
- FBX30 antibody
Images
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Anti-FBXO30 antibody (ab68224) at 1/500 dilution + human colon tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 82 kDa
Observed band size: 82 kDa
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-FBXO30 antibody (ab68224) at 1/500 dilution
Lane 1 : FBXO30 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 82 kDa
Observed band size: 82 kDa
Datasheets and documents
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Datasheet download
References (0)
ab68224 has not yet been referenced specifically in any publications.