Overview

  • Product name
  • Description
    Mouse polyclonal to FBXO31
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-367 of Human FBXO31

  • Positive control
    • FBXO31 transfected mammalian cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 66 kDa.

Target

  • Function
    Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in G1 arrest following DNA damage. Specifically recognizes phosphorylated cyclin-D1 (CCND1), promoting its ubiquitination and degradation by the proteasome, resulting in G1 arrest. May act as a tumor suppressor.
  • Tissue specificity
    Highly expressed in brain. Expressed at moderate levels in most tissues, except bone marrow.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the FBXO31 family.
    Contains 1 F-box domain.
  • Developmental stage
    Expression is cell-cycle regulated, and peaks at late G2 to early G1 phase (at protein level).
  • Post-translational
    modifications
    Phosphorylation at Ser-278 by ATM following gamma-irradiation results in its stabilization.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp434B027 antibody
    • DKFZp434J1815 antibody
    • F box only protein 31 antibody
    • F box protein 31 antibody
    • F-box only protein 31 antibody
    • FBX14 antibody
    • FBX31 antibody
    • FBX31_HUMAN antibody
    • FBXO14 antibody
    • Fbxo31 antibody
    • FLJ22477 antibody
    • MGC15419 antibody
    • MGC9527 antibody
    • pp2386 antibody
    • Putative breast cancer tumor suppressor antibody
    • SCF ubiquitin ligase specificity factor antibody
    see all

Images

  • All lanes : Anti-FBXO31 antibody (ab68067) at 1/500 dilution

    Lane 1 : FBXO31 (aa 1-367) transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugated at 1/2500 dilution

    Predicted band size : 66 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

References

ab68067 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab68067.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up