• Product name
  • Description
    Rabbit polyclonal to FBXO31
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within the amino acids 489-539 of Human FBXO31, NP_079011.3

  • Positive control
    • Whole cell lysate from 293T and mouse NIH3T3 cells.



Our Abpromise guarantee covers the use of ab86137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 24936062
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in G1 arrest following DNA damage. Specifically recognizes phosphorylated cyclin-D1 (CCND1), promoting its ubiquitination and degradation by the proteasome, resulting in G1 arrest. May act as a tumor suppressor.
    • Tissue specificity
      Highly expressed in brain. Expressed at moderate levels in most tissues, except bone marrow.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Belongs to the FBXO31 family.
      Contains 1 F-box domain.
    • Developmental stage
      Expression is cell-cycle regulated, and peaks at late G2 to early G1 phase (at protein level).
    • Post-translational
      Phosphorylation at Ser-278 by ATM following gamma-irradiation results in its stabilization.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp434B027 antibody
      • DKFZp434J1815 antibody
      • F box only protein 31 antibody
      • F box protein 31 antibody
      • F-box only protein 31 antibody
      • FBX14 antibody
      • FBX31 antibody
      • FBX31_HUMAN antibody
      • FBXO14 antibody
      • Fbxo31 antibody
      • FLJ22477 antibody
      • MGC15419 antibody
      • MGC9527 antibody
      • pp2386 antibody
      • Putative breast cancer tumor suppressor antibody
      • SCF ubiquitin ligase specificity factor antibody
      see all

    Anti-FBXO31 antibody images

    • All lanes : Anti-FBXO31 antibody (ab86137) at 0.4 µg/ml

      Lane 1 : Whole cell lysate from 293T cells
      Lane 2 : Whole cell lysate from mouse NIH3T3 cells

      Lysates/proteins at 50 µg per lane.

      Predicted band size : 61 kDa
      Observed band size : 61 kDa

      Exposure time : 30 seconds

    References for Anti-FBXO31 antibody (ab86137)

    This product has been referenced in:
    • Liu J  et al. F-box only protein 31 (FBXO31) negatively regulates p38 mitogen-activated protein kinase (MAPK) signaling by mediating lysine 48-linked ubiquitination and degradation of mitogen-activated protein kinase kinase 6 (MKK6). J Biol Chem 289:21508-18 (2014). ICC/IF ; Human . Read more (PubMed: 24936062) »

    See 1 Publication for this product

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