Overview

  • Product name
  • Description
    Rabbit polyclonal to FBXO4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 24-318 of Human FBXO4.

  • Positive control
    • HL60 whole cell lysates; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab153803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes ubiquitination of CCND1 and its subsequent proteasomal degradation. Recognizes TERF1 and promotes its ubiquitination together with UBE2D1.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 F-box domain.
  • Post-translational
    modifications
    Phosphorylation at Ser-12 varies during the cell cycle. It is low in resting cells and high in the S phase and the G2/M phase of the cell cycle. Phosphorylation is decreased during late G1 phase (By similarity). Phosphorylation at Ser-12 promotes homodimerization and is necessary for optimal ubiquitin ligase activity towards CCND1.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp547N213 antibody
    • F box only protein 4 antibody
    • F box protein 4 antibody
    • F box protein Fbx4 antibody
    • F-box only protein 4 antibody
    • FBX4 antibody
    • FBX4_HUMAN antibody
    • FBXO 4 antibody
    • FBXO4 antibody
    • FLJ10141 antibody
    see all

Images

  • Anti-FBXO4 antibody (ab153803) at 1/5000 dilution + HL60 whole cell
    lysate
    at 30 µg

    Predicted band size: 44 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling FBXO4 with ab153803 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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