Overview

  • Product name
  • Description
    Rabbit polyclonal to FbxO6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 48 and 293 of Human FBxO6 (Uniprot ID: Q9NRD1)

  • Positive control
    • 293T whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab153853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 34 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.

Target

  • Function
    Substrate-recognition component of some SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complexes. Involved in endoplasmic reticulum-associated degradation pathway (ERAD) for misfolded lumenal proteins by recognizing and binding sugar chains on unfolded glycoproteins that are retrotranlocated into the cytosol and promoting their ubiquitination and subsequent degradation. Able to recognize and bind denatured glycoproteins, which are modified with not only high-mannose but also complex-type oligosaccharides. Also recognizes sulfated glycans. Also involved in DNA damage response by specifically recognizing activated CHEK1 (phosphorylated on 'Ser-345'), promoting its ubiquitination and degradation. Ubiquitination of CHEK1 is required to insure that activated CHEK1 does not accumulate as cells progress through S phase, or when replication forks encounter transient impediments during normal DNA replication.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 F-box domain.
    Contains 1 FBA (F-box associated) domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • F box protein that recognizes sugar chains 2 antibody
    • F box G domain protein 2 antibody
    • F box only protein 6 antibody
    • F box protein 6 antibody
    • F box protein FBG2 antibody
    • F box protein Fbx6 antibody
    • F box/G domain protein 2 antibody
    • F-box only protein 6 antibody
    • F-box protein that recognizes sugar chains 2 antibody
    • F-box/G-domain protein 2 antibody
    • FBG 2 antibody
    • FBG2 antibody
    • FBS 2 antibody
    • FBS2 antibody
    • FBX 6 antibody
    • FBX6 antibody
    • FBX6_HUMAN antibody
    • FBXO 6 antibody
    • FBXO6 antibody
    • OTTHUMP00000002267 antibody
    see all

Images

  • Anti-FbxO6 antibody (ab153853) at 1/500 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 34 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Colon using ab153853 antibody at 1/500 dilution

References

ab153853 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab153853.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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