- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-FBXO7 antibody
See all FBXO7 primary antibodies -
DescriptionMouse polyclonal to FBXO7
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Host speciesMouse
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Full length protein corresponding to amino acids 1-522 of Human FBXO7 (AAH08361.1).
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Positive control
- Human placenta lysate and FBXO7 transfected 293T cell lysate. Human testis tissue. HeLa cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Constituent: 99% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab167278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa. | |
| IHC-P | Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
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FunctionSubstrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes BIRC2 and DLGAP5.
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PathwayProtein modification; protein ubiquitination.
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Involvement in diseaseDefects in FBXO7 are the cause of Parkinson disease type 15 (PARK15) [MIM:260300]; also known as parkinsonian-pyramidal syndrome. A neurodegenerative disorder characterized by parkinsonian and pyramidal signs. Clinical manifestations include tremor, bradykinesia, rigidity, postural instability, spasticity, mainly in the lower limbs, and hyperreflexia.
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Sequence similaritiesContains 1 F-box domain.
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Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
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Database links
- Entrez Gene: 25793 Human
- Omim: 605648 Human
- SwissProt: Q9Y3I1 Human
- Unigene: 5912 Human
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Alternative names
- F box only protein 7 antibody
- F box protein 7 antibody
- F-box only protein 7 antibody
see all
Images
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Western blot - Anti-FBXO7 antibody (ab167278)Anti-FBXO7 antibody (ab167278) at 1/500 dilution + Human placenta lysate at 25 µg
Developed using the ECL technique.
Predicted band size: 59 kDa -
Western blot - Anti-FBXO7 antibody (ab167278)All lanes : Anti-FBXO7 antibody (ab167278) at 1 µg/ml
Lane 1 : FBXO7 transfected 293T cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 15 µl per lane.
Developed using the ECL technique.
Predicted band size: 59 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO7 antibody (ab167278)Immunohistochemical analysis of formalin fixed, paraffin embedded Human testis tissue labeling FBXO7 with ab167278 at 3 µg/ml. -
Immunocytochemistry/ Immunofluorescence - Anti-FBXO7 antibody (ab167278)Immunofluorescence analysis of HeLa cells, labeling FBXO7 with ab167278 at 10 µg/ml.
Protocols
References
ab167278 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"