Overview

  • Product name
  • Description
    Mouse polyclonal to FBXO7
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to amino acids 1-522 of Human FBXO7 (AAH08361.1).

  • Positive control
    • Human placenta lysate and FBXO7 transfected 293T cell lysate. Human testis tissue. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab167278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes BIRC2 and DLGAP5.
  • Pathway
    Protein modification; protein ubiquitination.
  • Involvement in disease
    Defects in FBXO7 are the cause of Parkinson disease type 15 (PARK15) [MIM:260300]; also known as parkinsonian-pyramidal syndrome. A neurodegenerative disorder characterized by parkinsonian and pyramidal signs. Clinical manifestations include tremor, bradykinesia, rigidity, postural instability, spasticity, mainly in the lower limbs, and hyperreflexia.
  • Sequence similarities
    Contains 1 F-box domain.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • F box only protein 7 antibody
    • F box protein 7 antibody
    • F-box only protein 7 antibody
    • FBX antibody
    • FBX07 antibody
    • FBX7 antibody
    • FBX7_HUMAN antibody
    • Fbxo7 antibody
    • PARK15 antibody
    • PKPS antibody
    see all

Images

  • Anti-FBXO7 antibody (ab167278) at 1/500 dilution + Human placenta lysate at 25 µg
    Developed using the ECL technique

    Predicted band size : 59 kDa
  • All lanes : Anti-FBXO7 antibody (ab167278) at 1 µg/ml

    Lane 1 : FBXO7 transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 15 µl per lane.

    Developed using the ECL technique

    Predicted band size : 59 kDa
  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human testis tissue labeling FBXO7 with ab167278 at 3 µg/ml.
  • Immunofluorescence analysis of HeLa cells, labeling FBXO7 with ab167278 at 10 µg/ml.

References

ab167278 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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