Overview

  • Product nameAnti-FBXO7 antibody
    See all FBXO7 primary antibodies
  • Description
    Rabbit polyclonal to FBXO7
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within the middle amino acids 467-516 (

    PGETPSQFPPLRPRFDPVGPLPGPNPILPGRGGPNDRFPFRPSRGRPTDG

    ) of Human FBXO7 (NP_036311)

  • Positive control
    • MCF7 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab84129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionSubstrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes BIRC2 and DLGAP5.
  • PathwayProtein modification; protein ubiquitination.
  • Involvement in diseaseDefects in FBXO7 are the cause of Parkinson disease type 15 (PARK15) [MIM:260300]; also known as parkinsonian-pyramidal syndrome. A neurodegenerative disorder characterized by parkinsonian and pyramidal signs. Clinical manifestations include tremor, bradykinesia, rigidity, postural instability, spasticity, mainly in the lower limbs, and hyperreflexia.
  • Sequence similaritiesContains 1 F-box domain.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • F box only protein 7 antibody
    • F box protein 7 antibody
    • F-box only protein 7 antibody
    • FBX antibody
    • FBX07 antibody
    • FBX7 antibody
    • FBX7_HUMAN antibody
    • Fbxo7 antibody
    • PARK15 antibody
    • PKPS antibody
    see all

Anti-FBXO7 antibody images



  • Predicted band size : 59 kDa
    Western blot analysis of HeLa cell lysate labeling FBXO7 with ab84129 at 1.0µg/ml.


  • Predicted band size : 59 kDa
    Western blot analysis of 293T cell lysate labeling FBXO7 with ab84129 at 1.0µg/ml.
  • Anti-FBXO7 antibody (ab84129) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 59 kDa
    Observed band size : 59,70 kDa (why is the actual band size different from the predicted?)

References for Anti-FBXO7 antibody (ab84129)

ab84129 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 4°C
Sample Human Cell (HCC 1954 - Breast cancer cell lines)
Specification HCC 1954 - Breast cancer cell lines
Permeabilization Yes - 0.3% Triton X-100 in TBS
Fixative Formaldehyde
Username

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Verified customer

Submitted Dec 31 2014

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (Daoy)
Specification Daoy
Treatment 20 nM siRNA for 72 hours
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Username

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Verified customer

Submitted Nov 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"