Overview

  • Product nameAnti-FBXW10 antibody
  • Description
    Rabbit polyclonal to FBXW10
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 643-725 (LNGNCMKVLK ANGRGDPVLS FFIQGNRMVV NTESNVLMFQ FEHIKWQYAV EKTKQKKNKE KEEEKEENSL MEILSKCNIQ VHS) of Human FBXW10.

  • Positive control
    • Human smooth muscle tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionProbable substrate-recognition component of a SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Overexpression is leading to degradation of CBX5 and CBX1.
  • Sequence similaritiesContains 1 F-box domain.
    Contains 7 WD repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • F-box and WD-40 domain-containing protein 10 antibody
    • F-box/WD repeat-containing protein 10 antibody
    • FBW10_HUMAN antibody
    • FBXW10 antibody
    • Ubiquitin ligase-specificity factor antibody
    see all

Anti-FBXW10 antibody images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus and nucleoli. Recommended concentration of ab121720 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121720 at 1/125 dilution staining FBXW10 in Paraffin-embedded Human Smooth muscle tissue by Immunohistochemistry.

References for Anti-FBXW10 antibody (ab121720)

ab121720 has not yet been referenced specifically in any publications.

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