Recombinant
RabMAb

Anti-Fbxw7 antibody [EPR8069(2)] (ab171961)

Overview

  • Product name
    Anti-Fbxw7 antibody [EPR8069(2)]
    See all Fbxw7 primary antibodies
  • Description
    Rabbit monoclonal [EPR8069(2)] to Fbxw7
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Fbxw7 aa 650 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q969H0

  • Positive control
    • Human fetal heart, Human fetal muscle and Human small intestine lysates; HeLa cells
  • General notes

    Mouse, rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab171961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 79 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/20.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. Involved in the degradation of cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1.
    • Tissue specificity
      Isoform 1 is widely expressed. Isoform 4 is expressed in brain.
    • Sequence similarities
      Contains 1 F-box domain.
      Contains 7 WD repeats.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Cytoplasm; Nucleus; Nucleus > nucleolus and Nucleus > nucleoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • AGO antibody
      • Archipelago antibody
      • Archipelago homolog antibody
      • Archipelago, Drosophila, homolog of antibody
      • CDC4 antibody
      • CDC4, S.cerevisiae, homolog of antibody
      • DKFZp686F23254 antibody
      • F box and WD 40 domain protein 7 (archipelago homolog, Drosophila) antibody
      • F box and WD 40 domain protein 7 antibody
      • F box and WD repeat domain containing 7 antibody
      • F box protein FBW7 antibody
      • F box protein FBX30 antibody
      • F box protein SEL 10 antibody
      • F box protein SEL10 antibody
      • F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase antibody
      • F-box and WD-40 domain-containing protein 7 antibody
      • F-box protein FBX30 antibody
      • F-box/WD repeat-containing protein 7 antibody
      • FBW6 antibody
      • FBW7 antibody
      • FBX30 antibody
      • FBXO30 antibody
      • FBXW6 antibody
      • FBXW7 antibody
      • FBXW7_HUMAN antibody
      • FLJ16457 antibody
      • hAgo antibody
      • hCdc4 antibody
      • Homolog of C elegans sel 10 antibody
      • Homolog of C.elegans sel10 antibody
      • SEL 10 antibody
      • SEL-10 antibody
      • SEL10 antibody
      see all

    Images

    • Anti-Fbxw7 antibody [EPR8069(2)] (ab171961) at 1/1000 dilution (purified) + Human fetal heart at 10 µg

      Secondary
      Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 79 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunofluorescence staining of HeLa cells with purified ab171961 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab171961 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Fbxw7 with purified ab171961 at 1/20 dilution(10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor®488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    • Anti-Fbxw7 antibody [EPR8069(2)] (ab171961) at 1/5000 dilution (purified) + Human fetal muscle at 10 µg

      Secondary
      Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 79 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Fbxw7 antibody [EPR8069(2)] (ab171961) at 1/1000 dilution (Unpurified)

      Lane 1 : Human fetal heart lysate
      Lane 2 : Human fetal muscle lysate
      Lane 3 : Human small intestine lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 79 kDa
    • Immunofluorescent staining of HeLa cells labeling Fbxw7 with unpurified ab171961 at 1/100 dilution. 

    References

    This product has been referenced in:
    • Xu R  et al. The protease Omi regulates mitochondrial biogenesis through the GSK3ß/PGC-1a pathway. Cell Death Dis 5:e1373 (2014). WB ; Mouse . Read more (PubMed: 25118933) »
    • Izumi H & Kaneko Y Trim32 facilitates degradation of MYCN on spindle poles and induces asymmetric cell division in human neuroblastoma cells. Cancer Res 74:5620-30 (2014). Read more (PubMed: 25100564) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Nov 06 2017

    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (7.5%)
    Sample
    Mouse Tissue lysate - whole (liver)
    Specification
    liver
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5%
    Username

    Dr. 金录 郭

    Verified customer

    Submitted Nov 04 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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