The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. Involved in the degradation of cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1.
Isoform 1 is widely expressed. Isoform 4 is expressed in brain.
Contains 1 F-box domain. Contains 7 WD repeats.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cytoplasm; Nucleus; Nucleus > nucleolus and Nucleus > nucleoplasm.