Anti-Fc epsilon RI antibody [0.T.195] (ab33568)

Overview

  • Product nameAnti-Fc epsilon RI antibody [0.T.195]See all Fc epsilon RI primary antibodies ...
  • Description
    Mouse monoclonal [0.T.195] to Fc epsilon RI
  • SpecificityThis antibody recognizes Fc epsilon RI.
  • Tested applicationsICC/IF, Flow Cyt, IP more details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Whole rat basophilic leukemia cells, positive for the rat high affinity receptor recognizing immunoglobulin E (Fc epsilon RI).

  • Positive control
    • Biotinylated rat basophilic leukemia cell lysate.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 30% Glycerol, 0.15M Sodium chloride, 0.1M Tris glycine
  • Concentration information loading...
  • PurityProtein G purified
  • Clonality Monoclonal
  • Clone number0.T.195
  • IsotypeIgG1
  • Light chain typekappa
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab33568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.
IP Use at an assay dependent dilution. 5 µg immunoprecipitates Fc epsilon RI from 500 µg of cell lysate.

Target

  • FunctionBinds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.
  • Sequence similaritiesContains 2 Ig-like (immunoglobulin-like) domains.
  • Cellular localizationCell membrane.
  • Target information above from: UniProt accession P12319 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Fc epsilon RI alpha antibody
    • Fc epsilon RI alpha chain antibody
    • Fc epsilon RI alpha-chain antibody
    • Fc fragment of IgE high affinity I receptor for alpha polypeptide antibody
    • Fc fragment of IgE, high affinity I, receptor for, alpha subunit antibody
    • Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide antibody
    • Fc IgE receptor alpha polypeptide antibody
    • Fc IgE receptor, alpha chain antibody
    • Fc IgE receptor, alpha polypeptide antibody
    • Fc of IgE high affinity I receptor for alpha polypeptide antibody
    • Fc-epsilon RI-alpha antibody
    • FCE 1A antibody
    • FCE1A antibody
    • FCER 1A antibody
    • FCER1A antibody
    • FCERA_HUMAN antibody
    • FceRI alpha antibody
    • FcERI antibody
    • high affinity IgE receptor antibody
    • High affinity immunoglobulin epsilon receptor alpha subunit antibody
    • high affinity immunoglobulin epsilon receptor alpha-subunit antibody
    • High affinity immunoglobulin epsilon receptor subunit alpha antibody
    • IgE Fc receptor alpha subunit antibody
    • IgE Fc receptor subunit alpha antibody
    • Immunoglobulin E receptor high affinity of mast cells alpha polypeptide antibody
    • immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide antibody
    see all

Anti-Fc epsilon RI antibody [0.T.195] images

  • ab33568 staining Fc epsilon RI (red) in Rat mast cells (RBL) by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed and permeabilized in 0.15 Triton X-100 prior to blocking in 1% BSA and 1% FBS for 30 minutes at 22°C. The primary antibody was diluted 1/500 in PBS with 1% BSA and 1% FBS and incubated with the sample for 1 hour at 22°C. The secondary antibody was Alexa Fluor® 647-conjugated Goat anti-Mouse polyclonal, diluted 1/400.

    See Abreview

References for Anti-Fc epsilon RI antibody [0.T.195] (ab33568)

ab33568 has not yet been referenced specifically in any publications.

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We recommend storing the antibody at -20C after immediately after receiving it or after 1-2 weeks of ordering. We do not provide guarantee if the antibodies aren't stored as recommended. In your case the antibody was store...

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Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Mast cell - RBL)
Specification Mast cell - RBL
Fixative Paraformaldehyde
Permeabilization Yes - I have samples with and without permeabilization- 0.1%triton
Blocking step (agent) for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Ms. Nurit Pereg-Azouz

Verified customer

Submitted Mar 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"