The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 1µg for 105-8 cells.
(in a final volume of 100 μl).
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.
Flow cytometric analysis of Human lysed whole blood with Mouse IgG2b Isotype Control (green histogram) or 1 µg ab171278 (blue histogram) followed by Anti-Mouse IgG Biotin and Streptavidin PE. Cells in the lymphocyte gate were used for analysis.