Biotin Anti-Fc epsilon RI/FCER1A antibody [CRA1] (ab122882)
Key features and details
- Biotin Mouse monoclonal [CRA1] to Fc epsilon RI/FCER1A
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG2b
Overview
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Product name
Biotin Anti-Fc epsilon RI/FCER1A antibody [CRA1]
See all Fc epsilon RI/FCER1A primary antibodies -
Description
Biotin Mouse monoclonal [CRA1] to Fc epsilon RI/FCER1A -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Fc epsilon RI/FCER1A aa 1-100.
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Epitope
corresponds to Amino acids 1-84 of Human Fc epsilon RI/FCER1A -
Positive control
- CHO/alpha beta gamma cells
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General notes
[biotin]/[IgG] = 8.9
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Purification notes
The IgG fraction was purified from serum free culture medium of mouse hybridoma (CRA1) by propriety chromatography under mild conditions. Filter sterilized. -
Clonality
Monoclonal -
Clone number
CRA1 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122882 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines. -
Sequence similarities
Contains 2 Ig-like (immunoglobulin-like) domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2205 Human
- Omim: 147140 Human
- SwissProt: P12319 Human
- Unigene: 897 Human
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Alternative names
- Fc epsilon RI alpha antibody
- Fc epsilon RI alpha chain antibody
- Fc epsilon RI alpha-chain antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab122882 has been referenced in 4 publications.
- Untersmayr E et al. The high affinity IgE receptor Fc epsilonRI is expressed by human intestinal epithelial cells. PLoS One 5:e9023 (2010). PubMed: 20126404
- Takai T et al. Direct expression of the extracellular portion of human FcepsilonRIalpha chain as inclusion bodies in Escherichia coli. Biosci Biotechnol Biochem 65:79-85 (2001). PubMed: 11272849
- Takai T et al. Epitope analysis and primary structures of variable regions of anti-human FcepsilonRI monoclonal antibodies, and expression of the chimeric antibodies fused with human constant regions. Biosci Biotechnol Biochem 64:1856-67 (2000). PubMed: 11055388
- Yamaguchi M et al. IgE enhances Fc epsilon receptor I expression and IgE-dependent release of histamine and lipid mediators from human umbilical cord blood-derived mast cells: synergistic effect of IL-4 and IgE on human mast cell Fc epsilon receptor I expression and mediator release. J Immunol 162:5455-65 (1999). PubMed: 10228025