Overview

  • Product name
    Anti-Fc epsilon RI antibody [CRA2] (FITC)
    See all Fc epsilon RI primary antibodies
  • Description
    Mouse monoclonal [CRA2] to Fc epsilon RI (FITC)
  • Host species
    Mouse
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: ICC, Flow Cyt, Inhibition Assaymore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: FEDSGEYKCQ HQQVNESEPV YLEVFSDWLL LQASAEVVME GQPLFLRCHG WRNWDVYKVI YYKDGEALKY WYENHNISIT NATVEDSG, corresponding to amino acids 85-172 of Human Fc epsilon RI

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Epitope
    Amino acids 85-172 of Fc epsilon RI
  • Positive control
    • CHO/alpha beta gamma cells
  • General notes

    [FITC]/[IgG] 8.9

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.40
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity
    Ion Exchange Chromatography
  • Purification notes
    ab122891 was purified from serum free culture medium of mouse hybridoma (CRA2) by propriety chromatography under mild conditions. Filter sterilized
  • Clonality
    Monoclonal
  • Clone number
    CRA2
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab106163 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
Inhibition Assay Use at an assay dependent concentration. Inhibition of binding of IgE with Fc epsilon RI

Target

  • Function
    Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.
  • Sequence similarities
    Contains 2 Ig-like (immunoglobulin-like) domains.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • Fc epsilon RI alpha antibody
      • Fc epsilon RI alpha chain antibody
      • Fc epsilon RI alpha-chain antibody
      • Fc fragment of IgE high affinity I receptor for alpha polypeptide antibody
      • Fc fragment of IgE, high affinity I, receptor for, alpha subunit antibody
      • Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide antibody
      • Fc IgE receptor alpha polypeptide antibody
      • Fc IgE receptor, alpha chain antibody
      • Fc IgE receptor, alpha polypeptide antibody
      • Fc of IgE high affinity I receptor for alpha polypeptide antibody
      • Fc-epsilon RI-alpha antibody
      • FCE 1A antibody
      • FCE1A antibody
      • FCER 1A antibody
      • Fcer1a antibody
      • FCERA_HUMAN antibody
      • FceRI alpha antibody
      • FcERI antibody
      • high affinity IgE receptor antibody
      • High affinity immunoglobulin epsilon receptor alpha subunit antibody
      • high affinity immunoglobulin epsilon receptor alpha-subunit antibody
      • High affinity immunoglobulin epsilon receptor subunit alpha antibody
      • IgE Fc receptor alpha subunit antibody
      • IgE Fc receptor subunit alpha antibody
      • Immunoglobulin E receptor high affinity of mast cells alpha polypeptide antibody
      • immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide antibody
      see all

    Images

    • Flow Cytometry - Anti-Fc epsilon RI antibody [CRA2] (FITC) (ab122891)
      Flow Cytometry - Anti-Fc epsilon RI antibody [CRA2] (FITC) (ab122891)
      FACS analysis of CHO/alpha beta gamma cells (1x105) with CRA1 and CRA2 antibodies by indirect-immunostaining using FITC-labeled secondary antibody.

    References

    This product has been referenced in:
    • Takai T  et al. Direct expression of the extracellular portion of human FcepsilonRIalpha chain as inclusion bodies in Escherichia coli. Biosci Biotechnol Biochem 65:79-85 (2001). Read more (PubMed: 11272849) »
    • Goto T  et al. Enhanced expression of the high-affinity receptor for IgE (Fc(epsilon)RI) associated with decreased numbers of Langerhans cells in the lesional epidermis of atopic dermatitis. J Dermatol Sci 27:156-61 (2001). Read more (PubMed: 11641054) »

    See all 4 Publications for this product

    Publishing research using ab122891? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab122891.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up