Overview

  • Product nameFc-gamma RIIB/C Human ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 85 pg/ml
  • Range
    78.13 pg/ml - 5000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 88.89 79% - 99%
    Serum 100 91% - 108%
    Plasma 112.1 96% - 135%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Fc-gamma RIIB/C (Fc gamma Receptor II B/C) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Fc-gamma RIIB/C in serum, plasma, cell culture supernatants.

    This assay employs an antibody specific for Human Fc-gamma RIIB/C coated on a 96- well plate. Standards and samples are pipetted into the wells and Fc-gamma RIIB/C present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Fc-gamma RIIB/C antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fc-gamma RIIB/C bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab113333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

Fc-gamma RIIB/C Human ELISA Kit images

  • Representative Standard Curve using ab113333

  • Representative Standard Curve using ab113333

Protocols

References for Fc-gamma RIIB/C Human ELISA Kit (ab113333)

ab113333 has not yet been referenced specifically in any publications.

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