Overview

  • Product nameFCCP
  • Description
    Potent mitochondrial oxidative phosphorylation uncoupler
  • Biological descriptionPotent mitochondrial oxidative phosphorylation uncoupler (IC50 = 20 nM). Disrupts ATP synthesis by transporting protons across mitochondrial inner membranes. Depolarises mitochondrial membrane potential.
  • Purity> 99%

Properties

    References for FCCP (ab120081)

    This product has been referenced in:
    • Fossati G  et al. The mitochondrial network of human neutrophils: role in chemotaxis, phagocytosis, respiratory burst activation, and commitment to apoptosis. J Immunol 170:1964-72 (2003). Read more (PubMed: 12574365) »
    • Collins TJ  et al. Inositol 1,4,5-trisphosphate-induced Ca2+ release is inhibited by mitochondrial depolarization. Biochem J 347:593-600 (2000). Read more (PubMed: 10749691) »
    • Gautier I  et al. A moderate but not total decrease of mitochondrial membrane potential triggers apoptosis in neuron-like cells. Neuroreport 11:2953-6 (2000). Read more (PubMed: 11006972) »
    • Benz R & McLaughlin S The molecular mechanism of action of the proton ionophore FCCP (carbonylcyanide p-trifluoromethoxyphenylhydrazone). Biophys J 41:381-98 (1983). Read more (PubMed: 6838976) »

    See all 6 Publications for this product

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    The concentration of CCCP or FCCP required as a positive control will vary based on cell line. Perhaps it would be useful to try 50uM FCCP as this has worked for you in the past.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"