Overview

  • Product name
  • Description
    Rabbit polyclonal to FCGBP
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to internal sequence amino acids 289-417 of Human FCGBP.

  • Positive control
    • U 2 OS cells; Human rectum tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May be involved in the maintenance of the mucosal structure as a gel-like component of the mucosa.
  • Tissue specificity
    Mainly expressed in placenta and colon epithelium. Expressed in thyroid, and down-regulated in thyroid carcinomas. Present in serum, with higher levels in patients with various autoimmune diseases (at protein level).
  • Sequence similarities
    Contains 12 TIL (trypsin inhibitory-like) domains.
    Contains 13 VWFD domains.
  • Domain
    The N-terminal IgGFc-binding region is primate-specific.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fc fragment of IgG binding protein antibody
    • FC(GAMMA)BP antibody
    • Fcgamma-binding protein antigen antibody
    • FcgammaBP antibody
    • FCGBP antibody
    • FCGBP_HUMAN antibody
    • Human Fc gamma BP antibody
    • IgGFc-binding protein antibody
    see all

Images

  • ab121202 at 1/200 dilution staining FCGBP in Paraffin-embedded Human rectum tissue by Immunohistochemistry.
  • ab121202 at 4 µg/ml staining FCGBP in PFA/Triton X-100 fixed/permeabilized U 2 OS cells by Immunofluorescence (green).

References

ab121202 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Glioma cell line)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
30 µg
Specification
Glioma cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mrs. Vidya Nimbalkar

Verified customer

Submitted Apr 29 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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