Anti-FCGR1A antibody (ab104273)

Overview

  • Product nameAnti-FCGR1A antibody
    See all FCGR1A primary antibodies
  • Description
    Rabbit polyclonal to FCGR1A
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Dog, Human
  • Immunogen

    KLH conjugated synthetic peptide corresponding to a region within internal amino acids 225-255 from Human FCGR1A (P12314)

  • Positive control
    • NCI-H460 cell lysate, Human brain tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab104273 is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab104273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 43 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionHigh affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses.
  • Tissue specificityMonocyte/macrophage specific.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. FCGR1 family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Phosphorylated on serine residues.
  • Cellular localizationCell membrane. Stabilized at the cell membrane through interaction with FCER1G.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD64 antibody
    • CD64A antibody
    • Fc fragment of IgG high affinity Ia receptor (CD64) antibody
    • Fc fragment of IgG, high affinity Ia, receptor for antibody
    • Fc gamma receptor antibody
    • Fc gamma receptor I A1 antibody
    • Fc gamma RI antibody
    • Fc gamma RIA antibody
    • Fc-gamma RI antibody
    • Fc-gamma RIA antibody
    • FCG1 antibody
    • FcgammaRIa antibody
    • FCGR1 antibody
    • FCGR1_HUMAN antibody
    • FCGR1A antibody
    • FcRI antibody
    • FLJ18345 antibody
    • High affinity immunoglobulin gamma Fc receptor I antibody
    • IGFR1 antibody
    • IgG Fc receptor I antibody
    • Immunoglobulin G Fc receptor I antibody
    • OTTHUMP00000013913 antibody
    see all

Anti-FCGR1A antibody images

  • Anti-FCGR1A antibody (ab104273) at 1/100 dilution + NCI-H460 cell lysate at 35 µg

    Predicted band size : 43 kDa
  • ab104273, at a 1/50 dilution, staining FCGR1A in formalin fixed and paraffin embedded Human brain tissue, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References for Anti-FCGR1A antibody (ab104273)

ab104273 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH 6.0 in steamer for 30 minutes
Sample Dog Tissue sections (Brain tissue (microglia, astrocytes) and leukocyte)
Specification Brain tissue (microglia, astrocytes) and leukocyte
Permeabilization No
Fixative Formaldehyde
Username

Dr. Guilherme Dias de Melo

Verified customer

Submitted Jul 17 2013

Thank you for that feedback. I am pleased to hear that the replacement antibody has been working more successfully than the original anti-CD64 antibody.


I, like you, havenot been able tofind much referenceto CD64 beingexpressed in epith...

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I have issued the free of charge antibody ab104273, which should be with you on Monday. The order number is xxxxxx. I hope the suggestions that I have made if not the new antibody will help. I wish you well with your experiments. If you do cont...

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Thank you for providing that extra information, and sorry for the delay in getting back to you. I wanted to make sure that I could help you in the best way possible before responding. I have been in contact with the source of this antibody (ab11984...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"