Overview

  • Product name
  • Description
    Rabbit polyclonal to FCHO1
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 468-517 (SPENVEDSGL DSPSHAAPGP SPDSWVPRPG TPQSPPSCRA PPPEARGIRA) of Human FCHO1 (NP_055937).

  • Positive control
    • 293T cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-FCHO1 antibody (ab84740) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 97 kDa
    Observed band size : 97 kDa
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.
  • ab84740 staining FCHO1 in human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab84740 staining FCHO1 in human uterus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References

This product has been referenced in:
  • Umasankar PK  et al. A clathrin coat assembly role for the muniscin protein central linker revealed by TALEN-mediated gene editing. Elife 3:N/A (2014). Read more (PubMed: 25303365) »
  • Umasankar PK  et al. Distinct and separable activities of the endocytic clathrin-coat components Fcho1/2 and AP-2 in developmental patterning. Nat Cell Biol 14:488-501 (2012). Read more (PubMed: 22484487) »

See all 3 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab84740.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up