Overview

  • Product nameAnti-FCHO1 antibody
    See all FCHO1 primary antibodies
  • Description
    Rabbit polyclonal to FCHO1
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 468-517 (SPENVEDSGL DSPSHAAPGP SPDSWVPRPG TPQSPPSCRA PPPEARGIRA) of Human FCHO1 (NP_055937).

  • Positive control
    • 293T cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-FCHO1 antibody images

  • Anti-FCHO1 antibody (ab84740) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 97 kDa
    Observed band size : 97 kDa
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.
  • ab84740 staining FCHO1 in human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab84740 staining FCHO1 in human uterus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References for Anti-FCHO1 antibody (ab84740)

This product has been referenced in:
  • Umasankar PK  et al. A clathrin coat assembly role for the muniscin protein central linker revealed by TALEN-mediated gene editing. Elife 3:N/A (2014). Read more (PubMed: 25303365) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"