Anti-FCHSD2 antibody (ab122485)
Key features and details
- Rabbit polyclonal to FCHSD2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FCHSD2 antibody -
Description
Rabbit polyclonal to FCHSD2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: LENSSKVVRD YNLQLFLQEN AVFHKPQPFQ FQPCDSDTSR QLESETGTTE EHSLNKEARK WATRVAREHK NIVHQQRVLN DLECHGAAVS EQSRA, corresponding to amino acids of Human FCHSD2.
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Positive control
- Human pancreas tissue; RT 4, U 251 MG, Human liver and tonsil lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122485 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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WB |
1/250 - 1/500.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
1/250 - 1/500. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Tissue specificity
Liver, brain, heart, placenta, skeletal muscle, pancreas, lung and kidney. -
Sequence similarities
Contains 1 FCH domain.
Contains 2 SH3 domains. - Information by UniProt
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Database links
- Entrez Gene: 9873 Human
- SwissProt: O94868 Human
- Unigene: 577053 Human
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Alternative names
- Carom antibody
- FCH and double SH3 domains 2 antibody
- FCH and double SH3 domains protein 2 antibody
see all
Images
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Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus, plasma membrane and cytoskeleton (actin filaments). Recommended concentration of ab122485 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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All lanes : Anti-FCHSD2 antibody (ab122485) at 1/250 dilution
Lane 1 : RT 4 cell line lysate
Lane 2 : U 251 MG cell line lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique. -
ab122485, at 1/50, staining FCHSD2 in paraffin embedded Human pancreas tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab122485 has not yet been referenced specifically in any publications.