The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionInvolved in serum exerting lectin activity. Binds GlcNAc.
Tissue specificityPeripheral blood leukocytes. Also detected in spleen, lung, and thymus, may be due to the presence of tissue macrophages or trapped blood in these tissues. Not detected on lymphocytes and granulocytes.
Sequence similaritiesBelongs to the ficolin lectin family. Contains 1 collagen-like domain. Contains 1 fibrinogen C-terminal domain.
Cellular localizationSecreted. Found on the monocyte surface.