Overview

  • Product nameAnti-FCN1 antibody
    See all FCN1 primary antibodies
  • Description
    Mouse monoclonal to FCN1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment: SSELRVDLVD FEGNHQFAKY KSFKVADEAE KYKLVLGAFV GGSAGNSLTG HNNNFFSTKD QDNDVSSSNC AEKFQGAWWY ADCHASNLNG LYLMGPHESY, corresponding to amino acids 201-300 of Human FCN1 (NP_001994) with a 26 kDa tag.

  • Positive control
    • FCN1 transfected 293T cell lysate; Recombinant tagged human FCN1 fragment.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 35 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged FCN1 is approximately 0.3ng/ml when used as a capture antibody.

Target

  • FunctionInvolved in serum exerting lectin activity. Binds GlcNAc.
  • Tissue specificityPeripheral blood leukocytes. Also detected in spleen, lung, and thymus, may be due to the presence of tissue macrophages or trapped blood in these tissues. Not detected on lymphocytes and granulocytes.
  • Sequence similaritiesBelongs to the ficolin lectin family.
    Contains 1 collagen-like domain.
    Contains 1 fibrinogen C-terminal domain.
  • Cellular localizationSecreted. Found on the monocyte surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • Collagen/fibrinogen domain containing protein 1 antibody
    • Collagen/fibrinogen domain-containing protein 1 antibody
    • Fcn1 antibody
    • FCN1_HUMAN antibody
    • FCNM antibody
    • ficolin (collagen/fibrinogen domain containing) 1 antibody
    • Ficolin 1 antibody
    • Ficolin A antibody
    • Ficolin alpha antibody
    • Ficolin-1 antibody
    • Ficolin-A antibody
    • Ficolin-alpha antibody
    • M ficolin antibody
    • M-ficolin antibody
    see all

Anti-FCN1 antibody images

  • Anti-FCN1 antibody (ab77454) at 1 µg/ml + Recombinant tagged human FCN1 fragment at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 35 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-FCN1 antibody (ab77454) at 1 µg/ml

    Lane 1 : FCN1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 35 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

References for Anti-FCN1 antibody (ab77454)

ab77454 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab77454.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"