Recombinant Anti-FDFT1 antibody [EPR16481] (ab195046)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16481] to FDFT1
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-FDFT1 antibody [EPR16481]
See all FDFT1 primary antibodies -
Description
Rabbit monoclonal [EPR16481] to FDFT1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain and fetal spleen lysate. HepG2 whole cell lysate (ab7900). Mouse brain, rat spleen, rat kidney and RAW264.7 cell lysate. Human lung squamous carcinoma tissue. Human sebaceous carcinoma tissue. A431 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16481 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab195046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/70.
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IHC-P |
1/16000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000. Predicted molecular weight: 48 kDa.
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Notes |
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IP
1/70. |
IHC-P
1/16000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Predicted molecular weight: 48 kDa. |
Target
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Pathway
Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 1/3. -
Sequence similarities
Belongs to the phytoene/squalene synthase family. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 2222 Human
- Entrez Gene: 14137 Mouse
- Entrez Gene: 29580 Rat
- Omim: 184420 Human
- SwissProt: P37268 Human
- SwissProt: P53798 Mouse
- SwissProt: Q02769 Rat
- Unigene: 593928 Human
see all -
Alternative names
- DGPT antibody
- ERG9 antibody
- Farnesyl diphosphate farnesyltransferase antibody
see all
Images
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All lanes : Anti-FDFT1 antibody [EPR16481] (ab195046) at 1/10000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 48 kDa -
Anti-FDFT1 antibody [EPR16481] (ab195046) at 1/10000 dilution + HepG2 cell lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 48 kDa -
All lanes : Anti-FDFT1 antibody [EPR16481] (ab195046) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat kidney lysate
Lane 3 : Rat spleen lysate
Lane 4 : RAW264.7 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa -
Immunohistochemical analysis of paraffin-embedded human lung squamous carcinoma tissue sections labeling FDFT1 with ab195046 at a 1/16000 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human sebaceous carcinoma tissue sections labeling FDFT1 with ab195046 at a 1/16000 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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FDFT1 was immunoprecipitated from HepG2 whole cell extract with ab195046 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab195046 at 1/10000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: HepG2 whole cell extract (Input) 10 µg. Lane 2: ab195046 IP in HepG2 whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab195046 in HeLa whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (10)
ab195046 has been referenced in 10 publications.
- Coukos R et al. An engineered transcriptional reporter of protein localization identifies regulators of mitochondrial and ER membrane protein trafficking in high-throughput CRISPRi screens. Elife 10:N/A (2021). PubMed: 34414886
- Shao Y et al. An Original Ferroptosis-Related Gene Signature Effectively Predicts the Prognosis and Clinical Status for Colorectal Cancer Patients. Front Oncol 11:711776 (2021). PubMed: 34249766
- Weng ML et al. Fasting inhibits aerobic glycolysis and proliferation in colorectal cancer via the Fdft1-mediated AKT/mTOR/HIF1a pathway suppression. Nat Commun 11:1869 (2020). PubMed: 32313017
- Ye D et al. MicroRNA-125a-mediated regulation of the mevalonate signaling pathway contributes to high glucose-induced proliferation and migration of vascular smooth muscle cells. Mol Med Rep 22:165-174 (2020). PubMed: 32319638
- Gonzalez-Rivera JC et al. Post-transcriptional air pollution oxidation to the cholesterol biosynthesis pathway promotes pulmonary stress phenotypes. Commun Biol 3:392 (2020). PubMed: 32699268
- Miller-Vedam LE et al. Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clients. Elife 9:N/A (2020). PubMed: 33236988
- Volkmar N et al. The ER membrane protein complex promotes biogenesis of sterol-related enzymes maintaining cholesterol homeostasis. J Cell Sci 132:N/A (2019). PubMed: 30578317
- Tian S et al. Proteomic Analysis Identifies Membrane Proteins Dependent on the ER Membrane Protein Complex. Cell Rep 28:2517-2526.e5 (2019). PubMed: 31484065
- Zhang P et al. Intrinsic BET inhibitor resistance in SPOP-mutated prostate cancer is mediated by BET protein stabilization and AKT-mTORC1 activation. Nat Med 23:1055-1062 (2017). PubMed: 28805822
- Shimada K et al. Global survey of cell death mechanisms reveals metabolic regulation of ferroptosis. Nat Chem Biol 12:497-503 (2016). WB . PubMed: 27159577