Overview

  • Product nameAnti-FE65 antibody [4H324]
    See all FE65 primary antibodies
  • Description
    Mouse monoclonal [4H324] to FE65
  • Tested applicationsSuitable for: ICC, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Fusion protein:

    DLPAGWM RVQDTSGTYY WHIPTGTTQW EPPGRASPSQ GSS

    , corresponding to amino acids 254-293 of Mouse FE65 (WW domain).

  • Positive control
    • Lysates of mouse, rat and human Alzheimer's diseased brains.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab17469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
WB
IP
  • Application notes
    ICC: Use at an assay dependent dilution. FE65 can be detected in rat primary neurons fixed with 4% paraformaldehyde.
    IP: 5µl can immunoprecipitate FE65 from 500ug of rat brain homogenate.
    WB: 1/1000 - 1/5000 detects FE65 in homogenates prepared from whole mouse, rat and human Alzheimer's diseased brains. Predicted molecular weight: 77 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscription coregulator that can have both coactivator and corepressor functions. Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its trancription activation activity. Function in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s).
    • Tissue specificityHighly expressed in brain; strongly reduced in post-mortem elderly subjects with Alzheimer disease.
    • Sequence similaritiesContains 2 PID domains.
      Contains 1 WW domain.
    • Post-translational
      modifications
      Phosphorylated following nuclear translocation. Phosphorylation at Tyr-546 enhances the transcription activation activity and reduces the affinity with RASD1/DEXRAS1.
    • Cellular localizationCell membrane. Cytoplasm. Nucleus. Cell projection > growth cone. Colocalizes with TSHZ3 in axonal growth cone (By similarity). In normal conditions, it mainly localizes to the cytoplasm, while a small fraction is tethered to the cell membrane via its interaction with APP. Following exposure to DNA damaging agents, it is released from cell membrane and translocates to the nucleus. Nuclear translocation is under the regulation of APP. Colocalizes with TSHZ3 in the nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adaptor protein FE65a2 antibody
      • Amyloid beta (A4) precursor protein binding family B member 1 antibody
      • Amyloid beta A4 precursor protein binding family B antibody
      • Amyloid beta A4 precursor protein binding family B member 1 antibody
      • Amyloid beta A4 precursor protein-binding family B member 1 antibody
      • Amyloid beta precursor protein binding family B member 1 antibody
      • APBB 1 antibody
      • APBB1 antibody
      • APBB1_HUMAN antibody
      • FE 65 antibody
      • Fe65 protein antibody
      • Protein Fe65 antibody
      • RIR antibody
      • stat like protein antibody
      see all

    References for Anti-FE65 antibody [4H324] (ab17469)

    This product has been referenced in:

    See 1 Publication for this product

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