Anti-FE65 antibody (ab51980)
Key features and details
- Goat polyclonal to FE65
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
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Overview
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Product name
Anti-FE65 antibody
See all FE65 primary antibodies -
Description
Goat polyclonal to FE65 -
Host species
Goat -
Specificity
This antibody is expected to recognise both reported isoforms. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Peptide with sequence CGSLKPKRLGAHTP, from the C Terminus of the protein sequence (Human)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Notes |
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WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 100 kDa (observed in lysates of NIH/3T3 and HeLa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Transcription coregulator that can have both coactivator and corepressor functions. Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its trancription activation activity. Function in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s). -
Tissue specificity
Highly expressed in brain; strongly reduced in post-mortem elderly subjects with Alzheimer disease. -
Sequence similarities
Contains 2 PID domains.
Contains 1 WW domain. -
Post-translational
modificationsPhosphorylated following nuclear translocation. Phosphorylation at Tyr-546 enhances the transcription activation activity and reduces the affinity with RASD1/DEXRAS1. -
Cellular localization
Cell membrane. Cytoplasm. Nucleus. Cell projection > growth cone. Colocalizes with TSHZ3 in axonal growth cone (By similarity). In normal conditions, it mainly localizes to the cytoplasm, while a small fraction is tethered to the cell membrane via its interaction with APP. Following exposure to DNA damaging agents, it is released from cell membrane and translocates to the nucleus. Nuclear translocation is under the regulation of APP. Colocalizes with TSHZ3 in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 11785 Mouse
- SwissProt: Q9QXJ1 Mouse
- Unigene: 38469 Mouse
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Alternative names
- Adaptor protein FE65a2 antibody
- Amyloid beta (A4) precursor protein binding family B member 1 antibody
- Amyloid beta A4 precursor protein binding family B antibody
see all
Images
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Anti-FE65 antibody (ab51980) at 0.1 µg/ml + NIH/3T3 lysate in RIPA buffer at 35 µg
Observed band size: 100 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
Datasheets and documents
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Datasheet download
References (1)
ab51980 has been referenced in 1 publication.
- Authiat MM et al. Preferential Involvement of BRCA1/BARD1, Not Tip60/Fe65, in DNA Double-Strand Break Repair in Presenilin-1 P117L Alzheimer Models. Neural Plast 2022:3172861 (2022). PubMed: 35237315