Overview

  • Product nameAnti-FE65 antibody [EPR3538]
    See all FE65 primary antibodies
  • Description
    Rabbit monoclonal [EPR3538] to FE65
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in human FE65 (UniProt ID: O00213).

  • Positive control
    • SH-SY5Y cell lysate and Human brain tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab91650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC
  • Application notesICC: 1/50.
    IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. The use of an HRP/AP polymerized antibody. We have compared both the HRP-conjugated and the polymerized HRP and found stronger signals can be obtained with the polymerized antibody.

    WB: 1/2000 - 1/5000. Predicted molecular weight: 77 kDa.

    Is unsuitable for Flow Cyt or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscription coregulator that can have both coactivator and corepressor functions. Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its trancription activation activity. Function in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s).
    • Tissue specificityHighly expressed in brain; strongly reduced in post-mortem elderly subjects with Alzheimer disease.
    • Sequence similaritiesContains 2 PID domains.
      Contains 1 WW domain.
    • Post-translational
      modifications
      Phosphorylated following nuclear translocation. Phosphorylation at Tyr-546 enhances the transcription activation activity and reduces the affinity with RASD1/DEXRAS1.
    • Cellular localizationCell membrane. Cytoplasm. Nucleus. Cell projection > growth cone. Colocalizes with TSHZ3 in axonal growth cone (By similarity). In normal conditions, it mainly localizes to the cytoplasm, while a small fraction is tethered to the cell membrane via its interaction with APP. Following exposure to DNA damaging agents, it is released from cell membrane and translocates to the nucleus. Nuclear translocation is under the regulation of APP. Colocalizes with TSHZ3 in the nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adaptor protein FE65a2 antibody
      • Amyloid beta (A4) precursor protein binding family B member 1 antibody
      • Amyloid beta A4 precursor protein binding family B antibody
      • Amyloid beta A4 precursor protein binding family B member 1 antibody
      • Amyloid beta A4 precursor protein-binding family B member 1 antibody
      • Amyloid beta precursor protein binding family B member 1 antibody
      • APBB 1 antibody
      • APBB1 antibody
      • APBB1_HUMAN antibody
      • FE 65 antibody
      • Fe65 protein antibody
      • Protein Fe65 antibody
      • RIR antibody
      • stat like protein antibody
      see all

    Anti-FE65 antibody [EPR3538] images

    • Anti-FE65 antibody [EPR3538] (ab91650) at 1/2000 dilution + SH-SY5Ycell lysates at 10 µg

      Secondary
      HRP labelled goat anti-rabbit IgG at 1/1000 dilution

      Predicted band size : 77 kDa
    • ab91650, at 1/50 dilution, staining FE65 in formalin-fixed, paraffin-embedded Human brain tissue, by Immunohistochemistry.

    References for Anti-FE65 antibody [EPR3538] (ab91650)

    ab91650 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"