Overview

  • Product name
    Feline IL-8 ELISA Kit (Interleukin-8)
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    25 pg/ml
  • Range
    25 pg/ml - 6000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 89.71 82% - 97%
    Plasma 84.6 71% - 95%
    Cell culture media 118 100% - 149%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Cat
  • Product overview

    Abcam’s IL8 (Interleukin-8) Feline ELISA Kit (ab193688) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of felineIL8 in serum, plasma (feline IL8 concentration is pretty low in normal plasma, it may not be detected in this assay) and cell culture supernatant.

    This assay employs an antibody specific for feline IL8 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures IL8 present in the samples. The wells are washed and biotinylated anti-feline IL8 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of IL8 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent 1 x 15ml
    Biotinylated feline IL-8 detection antibody (lyophilized) 2 vials
    feline IL-8 Standards 2 vials
    Pre-coated feline IL-8 Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • Cellular localization
    Secreted
  • Alternative names
    • Alveolar macrophage chemotactic factor I
    • Beta endothelial cell derived neutrophil activating peptide
    • Beta thromboglobulin like protein
    • C-X-C motif chemokine 8
    • Chemokine (C-X-C motif) ligand 8
    • CXCL8
    • Emoctakin
    • GCP-1
    • GCP1
    • Granulocyte chemotactic protein 1
    • Interleukin-8
    • LECT
    • LUCT
    • Lung giant cell carcinoma derived chemotactic protein
    • Lymphocyte derived neutrophil activating peptide
    • LYNAP
    • MDNCF
    • MONAP
    • Monocyte-derived neutrophil chemotactic factor
    • Monocyte-derived neutrophil-activating peptide
    • NAF
    • NAP-1
    • Neutrophil-activating protein 1
    • Protein 3-10C
    • Small inducible cytokine subfamily B member 8
    • T-cell chemotactic factor
    • Tumor necrosis factor induced gene 1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Standard Curve using ab193688 IL-8 (Interleukin-8) Feline ELISA Kit.

    Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Protocols

References

ab193688 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab193688.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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