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|Sample type||Average %||Range|
|Serum||89.71||82% - 97%|
|Plasma||84.6||71% - 95%|
|Cell culture media||118||100% - 149%|
Abcam’s IL8 (Interleukin-8) Feline ELISA Kit (ab193688) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of felineIL8 in serum, plasma (feline IL8 concentration is pretty low in normal plasma, it may not be detected in this assay) and cell culture supernatant.
This assay employs an antibody specific for feline IL8 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures IL8 present in the samples. The wells are washed and biotinylated anti-feline IL8 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of IL8 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|300X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent||1 x 15ml|
|Biotinylated feline IL-8 detection antibody (lyophilized)||2 vials|
|feline IL-8 Standards||2 vials|
|Pre-coated feline IL-8 Microplate (12 strips x 8 wells)||1 unit|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab193688 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab193688 has not yet been referenced specifically in any publications.