Overview

  • Product name
  • Description
    Rabbit polyclonal to FEM 1B
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    DINYQDQIPRTLEE

    , corresponding to amino acids 609-622 of Human FEM 1B(Peptide available as ab6098.)

  • General notes
    Stable for one year.


    Fas and Tumor Necrosis Factor Receptor 1 (TNFR 1) are two prototype members in the death receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR 1 was recently identified and designated F1A alpha and FEM 1 beta. F1Aa/FEM1b is the homologue of C. elegans sex determining protein FEM 1. FEM 1/F1Aa is cleaved by CED-3 and caspase. FEM 1/F1Aa associates with CED-4 and its mammalian homologue Apaf 1. Overexpression of F1Aa induces apoptosis. F1Aa is therefore a novel member of the death receptor associated protein that mediates apoptosis. F1Aa is expressed in a variety of human and mouse tissues.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Fas and Tumor Necrosis Factor Receptor 1 (TNFR 1) are two prototype members in the death receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR 1 was recently identified and designated F1A alpha and FEM 1 beta. F1Aa/FEM1b is the homologue of C. elegans sex determining protein FEM 1. FEM 1/F1Aa is cleaved by CED-3 and caspase. FEM 1/F1Aa associates with CED-4 and its mammalian homologue Apaf 1. Overexpression of F1Aa induces apoptosis. F1Aa is therefore a novel member of the death receptor associated protein that mediates apoptosis. F1Aa is expressed in a variety of human and mouse tissues.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 70 kDa.Can be blocked with FEM 1B peptide (ab6098).
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. Involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. Also involved in glucose homeostasis in pancreatic islet. Functions as an adapter/mediator in replication stress-induced signaling that leads to the activation of CHEK1.
  • Tissue specificity
    Widely expressed. Highly expressed in testis. Weakly expressed in other tissues.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the fem-1 family.
    Contains 8 ANK repeats.
    Contains 1 TPR repeat.
  • Cellular localization
    Cytoplasm. Nucleus. In the nucleus, the protein level increased slightly after camptothecin (CPT) treatment. Associated with chromatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • F1A alpha antibody
    • F1A-alpha antibody
    • F1AA antibody
    • Fem-1-like death receptor-binding protein alpha antibody
    • Fem-1-like in apoptotic pathway protein alpha antibody
    • Fem1 homolog b antibody
    • FEM1 like death receptor binding protein antibody
    • FEM1-beta antibody
    • FEM1b antibody
    • FEM1B_HUMAN antibody
    • Protein fem-1 homolog B antibody
    see all

Images

  • Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse liver tissue labeling FEM 1B with Anti-FEM 1B antibody (ab3686) at 20μg/ml.


  • Predicted band size : 70 kDa

    Western blot analysis of F1A alpha in mouse (A) and rat (B) liver tissue lysates with anti-F1A alpha (ab3686) at 1 µg /ml.

    Western blot analysis of FEM 1B in mouse (A) and rat (B) liver tissue lysates with anti-FEM 1B (ab3686) at 1 µg /ml.
  • ab3686 at 5µg/ml staining of FEM 1B in mouse liver tissue by IHC

  • Immunofluorescence of F1A alpha in Mouse Liver cells using ab3686 at 20 ug/ml.

References

ab3686 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab3686.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up