Overview

  • Product nameAnti-FEN1 antibody
    See all FEN1 primary antibodies
  • Description
    Rabbit polyclonal to FEN1
  • Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Sheep, Horse, Cow, Pig, Chimpanzee, Gorilla, Chinese Hamster, Orangutan, Bat
  • Immunogen

    Synthetic peptide representing human Flap Structure-specific Endonuclease I encoded within exon 2 (LocusLink ID 2237). The peptide is derived from a portion of FEN1 that begins a few residues downstream from the I-domain as defined in Swiss-Prot entry P39748.

  • Positive control
    • HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB 1/5000 - 1/60000. Detects a band of approximately 47 kDa (predicted molecular weight: 42 kDa).
IP Use at 4-10 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionStructure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
  • Sequence similaritiesBelongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.
  • Post-translational
    modifications
    Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.
    Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA.
    Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA.
  • Cellular localizationNucleus > nucleolus. Nucleus > nucleoplasm. Mitochondrion. Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNase IV antibody
    • FEN-1 antibody
    • FEN1 antibody
    • FEN1_HUMAN antibody
    • Flap endonuclease 1 antibody
    • Flap structure specific endonuclease 1 antibody
    • Flap structure-specific endonuclease 1 antibody
    • hFEN-1 antibody
    • hFEN1 antibody
    • Maturation factor 1 antibody
    • MF1 antibody
    • Rad2 antibody
    see all

Anti-FEN1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling FEN1 with ab17994 at 1/500 (2µg/ml). Detection: DAB.


  • Predicted band size : 42 kDa

    ab17994 at the indicated concentrations, staining human FEN1 in (20µg) HeLa cell lysate by Western blot. Secondary antibody coonjugated to HRP used at 1/40000 (for 0.015 µg/ml anti-Fen1) or 1/60000 (for 1 and 0.1µg/ml anti-Fen1). Detection was by chemiluminescence with the indicated exposure.

    ab17994 at the indicated concentrations, staining human FEN1 in (20µg) HeLa cell lysate by Western blot. Secondary antibody coonjugated to HRP used at 1/40000 (for 0.015 µg/ml anti-Fen1) or 1/60000 (for 1 and 0.1µg/ml anti-Fen1). Detection was by chemiluminescence with the indicated exposure.
  • ab17994 (1/200) staining FEN1 in HeLa cells. Cells were fixed in Paraformaldehyde and counterstained with DAPI in order to highlight the nucleus. Please refer to abreview for further experimental details.

    See Abreview

References for Anti-FEN1 antibody (ab17994)

ab17994 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

Submitted Jun 27 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"