• Product nameAnti-FEN1 antibody
    See all FEN1 primary antibodies
  • Description
    Rabbit polyclonal to FEN1
  • Specificityab64908 detects endogenous levels of total FEN1 protein.
  • Tested applicationsSuitable for: ELISA, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from internal sequence of human FEN1.

  • Positive control
    • HeLa cells. Human breast carcinoma tissue. Extracts from Jurkat cells, 293 cells and HUVEC cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab64908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IHC-P 1/50 - 1/100.


  • FunctionStructure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
  • Sequence similaritiesBelongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.
  • Post-translational
    Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.
    Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA.
    Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA.
  • Cellular localizationNucleus > nucleolus. Nucleus > nucleoplasm. Mitochondrion. Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNase IV antibody
    • FEN-1 antibody
    • FEN1 antibody
    • FEN1_HUMAN antibody
    • Flap endonuclease 1 antibody
    • Flap structure specific endonuclease 1 antibody
    • Flap structure-specific endonuclease 1 antibody
    • hFEN-1 antibody
    • hFEN1 antibody
    • Maturation factor 1 antibody
    • MF1 antibody
    • Rad2 antibody
    see all

Anti-FEN1 antibody images

  • All lanes : Anti-FEN1 antibody (ab64908) at 1/500 dilution

    Lane 1 : extracts from Jurkat cells
    Lane 2 : extracts from 293 cells
    Lane 3 : extracts from HUVEC cells
    Lane 4 : extracts from HUVEC cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
    Additional bands at : 117 kDa,28 kDa,30 kDa,34 kDa,95 kDa. We are unsure as to the identity of these extra bands.
  • ab64908, at 1/50 dilution, staining human breast carcinoma by immunohistochemistry using paraffin-embedded tissue, in the absence (left) or presnce (right) of immunising peptide.
  • ab64908, at 1/500 dilution, staining HeLa cells by immunofluorescence in the absence (left) or presence (right) of immunising peptide.

References for Anti-FEN1 antibody (ab64908)

ab64908 has not yet been referenced specifically in any publications.

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