Overview

  • Product nameAnti-FEN1 antibody
    See all FEN1 primary antibodies
  • Description
    Rabbit polyclonal to FEN1
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FEN1.

    (Peptide available as ab73360.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: HeLa Whole Cell, Thymus (Rat) Tissue, Hela Whole Cell - Bleomycin Treated (40U/ml), HepG2 Whole Cell, E14 TG2A Day 3 Whole Cell (data not shown)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab70815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).
IHC-P Use a concentration of 1 µg/ml.

Target

  • FunctionStructure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
  • Sequence similaritiesBelongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.
  • Post-translational
    modifications
    Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.
    Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA.
    Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA.
  • Cellular localizationNucleus > nucleolus. Nucleus > nucleoplasm. Mitochondrion. Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNase IV antibody
    • FEN-1 antibody
    • FEN1 antibody
    • FEN1_HUMAN antibody
    • Flap endonuclease 1 antibody
    • Flap structure specific endonuclease 1 antibody
    • Flap structure-specific endonuclease 1 antibody
    • hFEN-1 antibody
    • hFEN1 antibody
    • Maturation factor 1 antibody
    • MF1 antibody
    • Rad2 antibody
    see all

Anti-FEN1 antibody images

  • All lanes : Anti-FEN1 antibody (ab70815) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Thymus (Rat) Tissue Lysate
    Lane 3 : Hela Whole Cell Lysate - Bleomycin Treated (40U/ml)
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 16 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ICC/IF image of ab70815 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70815, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • IHC image of FEN1 in Human Tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70815, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
  • Anti-FEN1 antibody (ab70815) at 1 µg/ml + Recombinant Human FEN1 protein (ab95382) at 0.001 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 150 seconds

References for Anti-FEN1 antibody (ab70815)

This product has been referenced in:
  • Lokanga RA  et al. Heterozygosity for a hypomorphic Polß mutation reduces the expansion frequency in a mouse model of the Fragile X-related disorders. PLoS Genet 11:e1005181 (2015). WB ; Mouse . Read more (PubMed: 25886163) »
  • Mohni KN  et al. DNA mismatch repair proteins are required for efficient herpes simplex virus 1 replication. J Virol 85:12241-53 (2011). WB ; Rat . Read more (PubMed: 21957315) »

See all 3 Publications for this product

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