Overview

  • Product nameAnti-FEN1 antibody
    See all FEN1 primary antibodies
  • Description
    Rabbit polyclonal to FEN1
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Sheep, Horse, Cow, Pig, Chimpanzee, Gorilla, Chinese Hamster, Orangutan, Bat
  • Immunogen

    Synthetic peptide, corresponding to a region within the amino acids 250-300 of Human FEN1, NP_004102.1

  • Positive control
    • Human breast carcinoma, small cell lung cancer, testicular seminoma
  • General notesAntibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Overnight incubation at room temperature is recommended.

    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionStructure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
    • Sequence similaritiesBelongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.
    • Post-translational
      modifications
      Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.
      Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA.
      Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA.
    • Cellular localizationNucleus > nucleolus. Nucleus > nucleoplasm. Mitochondrion. Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.
    • Information by UniProt
    • Database links
    • Alternative names
      • DNase IV antibody
      • FEN-1 antibody
      • FEN1 antibody
      • FEN1_HUMAN antibody
      • Flap endonuclease 1 antibody
      • Flap structure specific endonuclease 1 antibody
      • Flap structure-specific endonuclease 1 antibody
      • hFEN-1 antibody
      • hFEN1 antibody
      • Maturation factor 1 antibody
      • MF1 antibody
      • Rad2 antibody
      see all

    Anti-FEN1 antibody images

    • Detection of Human FEN1 by Immunohistochemistry.
      Sample: FFPE section of Human small cell lung cancer.
      Antibody: ab84760 used at a dilution of 1/250.
      Detection: DAB staining.
    • Detection of Human FEN1 by Immunohistochemistry.
      Sample: FFPE section of Human testicular seminoma.
      Antibody: ab84760 used at a dilution of 1/250.
      Detection: DAB staining.

    References for Anti-FEN1 antibody (ab84760)

    ab84760 has not yet been referenced specifically in any publications.

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"