• Product nameAnti-FEN1 antibody [EPR4460(2)]
    See all FEN1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4460(2)] to FEN1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human FEN1.

  • Positive control
    • Jurkat, HeLa, Y79, and Daudi cell lysates, Human testis, Human tonsil, and Human urothelial carcinoma tissues
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab109132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P 1/500 - 1/750. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • FunctionStructure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
    • Sequence similaritiesBelongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.
    • Post-translational
      Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.
      Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA.
      Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA.
    • Cellular localizationNucleus > nucleolus. Nucleus > nucleoplasm. Mitochondrion. Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.
    • Information by UniProt
    • Database links
    • Alternative names
      • DNase IV antibody
      • FEN-1 antibody
      • FEN1 antibody
      • FEN1_HUMAN antibody
      • Flap endonuclease 1 antibody
      • Flap structure specific endonuclease 1 antibody
      • Flap structure-specific endonuclease 1 antibody
      • hFEN-1 antibody
      • hFEN1 antibody
      • Maturation factor 1 antibody
      • MF1 antibody
      • Rad2 antibody
      see all

    Anti-FEN1 antibody [EPR4460(2)] images

    • All lanes : Anti-FEN1 antibody [EPR4460(2)] (ab109132) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Y79 cell lysate
      Lane 4 : Daudi cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 42 kDa
    • Immunohistochemical analysis of paraffin-embedded Human testis tissue using ab109132 at a dilution of 1/500.
    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab109132 at a dilution of 1/500.
    • Immunohistochemical analysis of paraffin-embedded Human urothelial carcinoma tissue using ab109132 at a dilution of 1/500.

    References for Anti-FEN1 antibody [EPR4460(2)] (ab109132)

    ab109132 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab109132.
    Please use the links above to contact us or submit feedback about this product.