Overview

  • Product name
    Anti-FEN1 antibody [EPR4460(2)]
    See all FEN1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4460(2)] to FEN1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human FEN1.

  • Positive control
    • Jurkat, HeLa, Y79, and Daudi cell lysates, Human testis, Human tonsil, and Human urothelial carcinoma tissues
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P 1/500 - 1/750. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
    • Sequence similarities
      Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.
    • Post-translational
      modifications
      Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.
      Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA.
      Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA.
    • Cellular localization
      Nucleus > nucleolus. Nucleus > nucleoplasm. Mitochondrion. Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.
    • Information by UniProt
    • Database links
    • Alternative names
      • DNase IV antibody
      • FEN-1 antibody
      • FEN1 antibody
      • FEN1_HUMAN antibody
      • Flap endonuclease 1 antibody
      • Flap structure specific endonuclease 1 antibody
      • Flap structure-specific endonuclease 1 antibody
      • hFEN-1 antibody
      • hFEN1 antibody
      • Maturation factor 1 antibody
      • MF1 antibody
      • Rad2 antibody
      see all

    Images

    • All lanes : Anti-FEN1 antibody [EPR4460(2)] (ab109132) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Y79 cell lysate
      Lane 4 : Daudi cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 42 kDa
    • Immunohistochemical analysis of paraffin-embedded Human testis tissue using ab109132 at a dilution of 1/500.
    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab109132 at a dilution of 1/500.
    • Immunohistochemical analysis of paraffin-embedded Human urothelial carcinoma tissue using ab109132 at a dilution of 1/500.

    References

    This product has been referenced in:
    • Ward TA  et al. Small molecule inhibitors uncover synthetic genetic interactions of human flap endonuclease 1 (FEN1) with DNA damage response genes. PLoS One 12:e0179278 (2017). WB ; Human . Read more (PubMed: 28628639) »
    • Exell JC  et al. Cellularly active N-hydroxyurea FEN1 inhibitors block substrate entry to the active site. Nat Chem Biol 12:815-21 (2016). WB ; Human . Read more (PubMed: 27526030) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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