Overview

  • Product name
  • Description
    Goat polyclonal to FENS1
  • Specificity
    This antibody is expected to recognise both reported isoforms (as represented by NP_065881 and NP_848127).
  • Tested applications
    Suitable for: ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    VVGCSLATGFSPH

    , corresponding to C terminal amino acids 399-410 of Human FENS1

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab39274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration. Peptide ELISA: antibody detection limit dilution 1:32000
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Sequence similarities
    Contains 1 FYVE-type zinc finger.
    Contains 7 WD repeats.
  • Domain
    The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
  • Cellular localization
    Early endosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • FENS 1 antibody
    • FENS-1 antibody
    • FENS1 antibody
    • KIAA1435 antibody
    • Phosphoinositide binding protein 1 antibody
    • Phosphoinositide binding protein SR1 antibody
    • Phosphoinositide-binding protein 1 antibody
    • WD repeat and FYVE domain containing 1 antibody
    • WD repeat and FYVE domain-containing protein 1 antibody
    • WD40 and FYVE domain containing protein 1 antibody
    • WD40- and FYVE domain-containing protein 1 antibody
    • WDF1 antibody
    • WDFY1 antibody
    • WDFY1_HUMAN antibody
    • ZFYVE17 antibody
    • Zinc finger FYVE domain containing protein 17 antibody
    • Zinc finger FYVE domain-containing protein 17 antibody
    see all

Images

  • ab39274 (4µg/ml) staining of paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatoicytes. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

References

ab39274 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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