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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium Azide
Concentration information loading...
Purity Protein G purified
Purification notes ab71682 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 100 kDa (predicted molecular weight: 93 kDa).
Function Tyrosine kinase of the non-receptor type. Probably performs an important function, perhaps in regulatory processes such as cell cycle control.
Tissue specificity Expressed in a variety of lymphoid cell lines.
Sequence similarities Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily. Contains 1 FCH domain. Contains 1 protein kinase domain. Contains 1 SH2 domain.
Cellular localization Cytoplasm. Nucleus. Associated with the chromatin.
Information by UniProt
c FER antibody
Feline encephalitis virus related kinase FER antibody
Fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94) antibody
Anti-FER antibody - C-terminal images
Western blot - FER antibody - C-terminal (ab71682)
Anti-FER antibody - C-terminal (ab71682) at 1/100 dilution + HL-60 cell lysate at 12.5 µg Predicted band size : 93 kDa Observed band size : 100 kDa ( why is the actual band size different from the predicted?) Additional bands at : 50 kDa,75 kDa. We are unsure as to the identity of these extra bands.
References for Anti-FER antibody - C-terminal (ab71682)
This product has been referenced in:
et al. The Fer tyrosine kinase cooperates with interleukin-6 to activate signal transducer and activator of transcription 3 and promote human prostate cancer cell growth. Mol Cancer Res 7:142-55 (2009).
Read more (PubMed: 19147545) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"