Overview

  • Product nameAnti-FER antibody [EP1842Y]
    See all FER primary antibodies
  • Description
    Rabbit monoclonal [EP1842Y] to FER
  • SpecificityThis antibody reacts with FER
  • Tested applicationsSuitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues on the N terminus of FER (Human)

  • Positive control
    • Jurkat cell lysate
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab52479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).
IP Use a concentration of 5 µg/ml.
Flow Cyt 1/50. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC or IHC-P.
  • Target

    • FunctionTyrosine kinase of the non-receptor type. Probably performs an important function, perhaps in regulatory processes such as cell cycle control.
    • Tissue specificityExpressed in a variety of lymphoid cell lines.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.
      Contains 1 FCH domain.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
    • Cellular localizationCytoplasm. Nucleus. Associated with the chromatin.
    • Information by UniProt
    • Database links
    • Alternative names
      • c FER antibody
      • Feline encephalitis virus related kinase FER antibody
      • Fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94) antibody
      • Fer (fps/fes related) tyrosine kinase antibody
      • FER antibody
      • FER_HUMAN antibody
      • FerT antibody
      • Fujinami poultry sarcoma/Feline sarcoma related protein Fer antibody
      • p94 FER antibody
      • p94-FER antibody
      • Pe1Fe10 antibody
      • Pe1Fe13 antibody
      • Pe1Fe3 antibody
      • Pe1Fe6 antibody
      • Phosphoprotein NCP94 antibody
      • PPP1R74 antibody
      • Protein phosphatase 1 regulatory subunit 74 antibody
      • Proto oncogene tyrosine protein kinase FER antibody
      • Proto-oncogene c-Fer antibody
      • TYK3 antibody
      • Tyrosine kinase 3 antibody
      • Tyrosine-protein kinase Fer antibody
      see all

    Anti-FER antibody [EP1842Y] images



    • Predicted band size : 93 kDa

      Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: FER knockout HAP1 cell lysate (40 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab52479 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.

    • Anti-FER antibody [EP1842Y] (ab52479) at 1/5000 dilution + Jurkat cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 93 kDa
      Observed band size : 93 kDa
    • Overlay histogram showing Jurkat cells stained with ab52479 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52479, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • FER was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to FER and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
      The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab52479.
      Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
      Band: 93kDa; FER

    References for Anti-FER antibody [EP1842Y] (ab52479)

    This product has been referenced in:
    • McGinnis LK  et al. Fer tyrosine kinase is required for germinal vesicle breakdown and meiosis-I in mouse oocytes. Mol Reprod Dev 78:33-47 (2011). IHC-P, IF ; Mouse . Read more (PubMed: 21268181) »
    • Whitehead SN  et al. Transient and bilateral increase in Neuropilin-1, Fer kinase and collapsin response mediator proteins within membrane rafts following unilateral occlusion of the middle cerebral artery in mouse. Brain Res 1344:209-16 (2010). WB ; Mouse . Read more (PubMed: 20493826) »

    See all 3 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab52479.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"