Overview

  • Product nameAnti-FER (phospho Y402) antibody
    See all FER primary antibodies
  • Description
    Rabbit polyclonal to FER (phospho Y402)
  • Specificityab79573 detects endogenous levels of FER only when phosphorylated at tyrosine 402.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human FER around the phosphorylation site of tyrosine 402 (V-N-YP-E-E)

  • Positive control
    • Human brain tissue; Jurkat cell extracts starved for 24 hours.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab79573 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 93 kDa.
ELISA 1/20000.
IHC-P 1/50 - 1/100.

Target

  • FunctionTyrosine kinase of the non-receptor type. Probably performs an important function, perhaps in regulatory processes such as cell cycle control.
  • Tissue specificityExpressed in a variety of lymphoid cell lines.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.
    Contains 1 FCH domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
  • Cellular localizationCytoplasm. Nucleus. Associated with the chromatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • c FER antibody
    • Feline encephalitis virus related kinase FER antibody
    • Fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94) antibody
    • Fer (fps/fes related) tyrosine kinase antibody
    • FER antibody
    • FER_HUMAN antibody
    • FerT antibody
    • Fujinami poultry sarcoma/Feline sarcoma related protein Fer antibody
    • p94 FER antibody
    • p94-FER antibody
    • Pe1Fe10 antibody
    • Pe1Fe13 antibody
    • Pe1Fe3 antibody
    • Pe1Fe6 antibody
    • Phosphoprotein NCP94 antibody
    • PPP1R74 antibody
    • Protein phosphatase 1 regulatory subunit 74 antibody
    • Proto oncogene tyrosine protein kinase FER antibody
    • Proto-oncogene c-Fer antibody
    • TYK3 antibody
    • Tyrosine kinase 3 antibody
    • Tyrosine-protein kinase Fer antibody
    see all

Anti-FER (phospho Y402) antibody images

  • ab79573, at a 1/50 dilution, staining FER in paraffin embedded human brain tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.
  • All lanes : Anti-FER (phospho Y402) antibody (ab79573) at 1/500 dilution

    Lane 1 : Jurkat cell extracts, starved (24 hours)
    Lane 2 : Jurkat cell extracts, starved (24 hours) with immunizing phospho peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 93 kDa
    Observed band size : 93 kDa
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

References for Anti-FER (phospho Y402) antibody (ab79573)

This product has been referenced in:
  • Chang SF  et al. Different modes of endothelial-smooth muscle cell interaction elicit differential ß-catenin phosphorylations and endothelial functions. Proc Natl Acad Sci U S A 111:1855-60 (2014). Read more (PubMed: 24449884) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"