Purification notesab79573 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Feline encephalitis virus related kinase FER antibody
Fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94) antibody
Fer (fps/fes related) tyrosine kinase antibody
Fujinami poultry sarcoma/Feline sarcoma related protein Fer antibody
p94 FER antibody
Phosphoprotein NCP94 antibody
Protein phosphatase 1 regulatory subunit 74 antibody
Proto oncogene tyrosine protein kinase FER antibody
Proto-oncogene c-Fer antibody
Tyrosine kinase 3 antibody
Tyrosine-protein kinase Fer antibody
Anti-FER (phospho Y402) antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FER (phospho Y402) antibody (ab79573)
ab79573, at a 1/50 dilution, staining FER in paraffin embedded human brain tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.
Western blot - FER (phospho Y402) antibody (ab79573)
All lanes : Anti-FER (phospho Y402) antibody (ab79573) at 1/500 dilution
Lane 1 : Jurkat cell extracts, starved (24 hours) Lane 2 : Jurkat cell extracts, starved (24 hours) with immunizing phospho peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 93 kDa Observed band size : 93 kDa Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
References for Anti-FER (phospho Y402) antibody (ab79573)
This product has been referenced in:
Chang SF et al. Different modes of endothelial-smooth muscle cell interaction elicit differential ß-catenin phosphorylations and endothelial functions. Proc Natl Acad Sci U S A111:1855-60 (2014).
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