Overview

  • Product nameFerritin (FTL) Human ELISA KitSee all Ferritin kits ...
  • Detection methodColorimetric
  • Precision
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.2%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Milk, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 1.5 ng/ml
  • Range
    1.563 ng/ml - 50 ng/ml
  • Recovery

    97 %

  • Assay time
    4h 0m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Monkey
  • Product overview

    Abcam’s Ferritin (FTL) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Ferritin concentrations in plasma, serum, milk, cerebrospinal fluid and cell culture supernatants.

    A Ferritin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Ferritin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Ferritin captured in plate.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionStores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
  • Involvement in diseaseDefects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
    Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.
  • Sequence similaritiesBelongs to the ferritin family.
    Contains 1 ferritin-like diiron domain.
  • Target information above from: UniProt accession P02792 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • ferritin L chain
    • Ferritin L subunit
    • Ferritin light chain
    • ferritin light polypeptide
    • ferritin light polypeptide like 3
    • FRIL_HUMAN
    • FTL
    • NBIA3
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent dilution.

Ferritin (FTL) Human ELISA Kit images

  • Representative Standard Curve using ab108837

Protocols

References for Ferritin (FTL) Human ELISA Kit (ab108837)

ab108837 has not yet been referenced specifically in any publications.

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The proper value in human plasma is 8-400 ng/ml. On average we get 60 ng/ml for our samples.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more ad...

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I can confirm that this kit is suitable for EDTA collected plasma.

ab108698 Ferritin Human ELISA Kit: for pre-treatment of serum samples we recommend HEPES buffer.

ab108837 Ferritin Human ELISA KIt: I am sorry this is not tested with other buffers, so we would not be able to advise which buffer to use. Ther...

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One immunogen is a recombinant protein and the other one is a purified protein.

Thank you very much for your reply. My colleague Jackie is out of the office today but I'll be happy to help you with this.

Do you need antibodies that are specific for ferritin heavy or ferritin light chains? Looking through our catalog, I o...

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Thank you for contacting us. We have a kit that may be suitable for your requirements. Ferritin Human ELISA KIt (ab108837) is designed for detection of Human Ferritin in plasma, serum, milk, and cell culture supernatants. This assay employs a quant...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"