Recombinant
RabMAb

Anti-Ferritin Heavy Chain antibody [EPR18878] (ab183781)

Overview

  • Product name
    Anti-Ferritin Heavy Chain antibody [EPR18878]
    See all Ferritin Heavy Chain primary antibodies
  • Description
    Rabbit monoclonal [EPR18878] to Ferritin Heavy Chain
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Mouse Ferritin Heavy Chain aa 50-150. The exact sequence is proprietary.
    Database link: P09528

  • Positive control
    • WB: Mouse liver and rat liver lysates; Mouse brain, heart and spleen lysates; Rat brain, kidney and spleen lysates; C6, RAW 264.7 and PC-12 whole cell lysates. IHC-P: Mouse liver and kidney tissues; Rat liver tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab183781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • Apoferritin antibody
    • Cell proliferation inducing gene 15 protein antibody
    • Cell proliferation-inducing gene 15 protein antibody
    • F HC antibody
    • Ferritin H subunit antibody
    • Ferritin heavy chain antibody
    • Ferritin heavy polypeptide 1 antibody
    • FHC antibody
    • FRIH antibody
    • FRIH_HUMAN antibody
    • FTH 1 antibody
    • FTH antibody
    • FTH1 antibody
    • FTH1 protein antibody
    • FTHL 6 antibody
    • FTHL6 antibody
    • Iron overload autosomal dominant antibody
    • MGC104426 antibody
    • N-terminally processed antibody
    • OK/SW-cl.84 antibody
    • PIG 15 antibody
    • PIG15 antibody
    • Placenta immunoregulatory factor antibody
    • PLIF antibody
    • Proliferation inducing gene 15 protein antibody
    • Proliferation inducing protein 15 antibody
    see all

Images

  • All lanes : Anti-Ferritin Heavy Chain antibody [EPR18878] (ab183781) at 1/1000 dilution

    Lane 1 : Mouse liver lysate
    Lane 2 : Rat liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size : 21 kDa
    Observed band size : 21 kDa


    Exposure time : 30 seconds

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Ferritin Heavy Chain antibody [EPR18878] (ab183781) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Rat brain lysate
    Lane 5 : Rat kidney lysate
    Lane 6 : Rat spleen lysate
    Lane 7 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 8 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 9 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size : 21 kDa
    Observed band size : 21 kDa

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time:

    Lane 1: 30 seconds
    Lanes 2-9: 3 minutes.

  • Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Ferritin Heavy Chain with ab183781 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on hepatic cell of the mouse liver is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Ferritin Heavy Chain with ab183781 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on the mouse kidney is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Ferritin Heavy Chain with ab183781 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on hepatic cell of the rat liver is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

References

ab183781 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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