Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)

Overview

  • Product nameAnti-Ferritin Heavy Chain antibody [EPR3005Y]
    See all Ferritin Heavy Chain primary antibodies
  • Description
    Rabbit monoclonal [EPR3005Y] to Ferritin Heavy Chain
  • SpecificityThis antibody recognizes only the Ferritin heavy chain.
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Frmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    corresponding to Human Ferritin Heavy Chain aa 150-250 (C terminal).
    Database link: P02794

  • Positive control
    • WB: Human fetal liver, SH-SY5Y, human brain and HeLa cell lysates. IHC: Human kidney, breast and tonsil tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

Properties

Applications

Our Abpromise guarantee covers the use of ab75972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration. PubMed: 25498860
  • Application notesIs unsuitable for ICC/IF.
  • Target

    • Information by UniProt
    • Database links
    • Alternative names
      • Apoferritin antibody
      • Cell proliferation inducing gene 15 protein antibody
      • Cell proliferation-inducing gene 15 protein antibody
      • F HC antibody
      • Ferritin H subunit antibody
      • Ferritin heavy chain antibody
      • Ferritin heavy polypeptide 1 antibody
      • FHC antibody
      • FRIH antibody
      • FRIH_HUMAN antibody
      • FTH 1 antibody
      • FTH antibody
      • FTH1 antibody
      • FTH1 protein antibody
      • FTHL 6 antibody
      • FTHL6 antibody
      • Iron overload autosomal dominant antibody
      • MGC104426 antibody
      • N-terminally processed antibody
      • OK/SW-cl.84 antibody
      • PIG 15 antibody
      • PIG15 antibody
      • Placenta immunoregulatory factor antibody
      • PLIF antibody
      • Proliferation inducing gene 15 protein antibody
      • Proliferation inducing protein 15 antibody
      see all

    Anti-Ferritin Heavy Chain antibody [EPR3005Y] images

    • Unpurified ab75972 showing positive staining in normal breast tissue.

    • ab75972 staining Ferritin Heavy Chain in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

      Negative control 1: PBS in place of primary antibody.

       

    • Lanes 1 - 3 : Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/500 dilution (unpurified)
      Lane 4 : Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/2000 dilution (unpurified)
      Lane 5 : Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/5000 dilution (unpurified)

      Lane 1 : Human liver lysate
      Lane 2 : SH-SY5Y lysate
      Lane 3 : Human brain lysate
      Lane 4 : Human brain lysate
      Lane 5 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 21 kDa
      Observed band size : 21 kDa
    • Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/5000 dilution + Human fetal liver at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 21 kDa
    • Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/2000 dilution + HeLa (human cervix adenocarcinoma) whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

      Predicted band size : 21 kDa
    • Unpurified ab75972 at 1/100 dilution staining Ferritin Heavy Chain in human kidney tissue, using a HRP/AP polymerized secondary antibody.

    • Unpurified ab75972 showing positive staining in normal liver tissue.

    • Unpurified ab75972 showing positive staining in normal tonsil tissue.

    References for Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)

    This product has been referenced in:
    • Vannucci L  et al. In Vivo Targeting of Cutaneous Melanoma Using an Melanoma Stimulating Hormone-Engineered Human Protein Cage with Fluorophore and Magnetic Resonance Imaging Tracers. J Biomed Nanotechnol 11:81-92 (2015). IHC-P ; Human . Read more (PubMed: 26301302) »
    • Yang H  et al. Upregulation of mitochondrial ferritin by proinflammatory cytokines: implications for a role in Alzheimer's disease. J Alzheimers Dis 45:797-811 (2015). Read more (PubMed: 25624418) »

    See all 6 Publications for this product

    Product Wall

    Application Flow Cytometry
    Sample Mouse Cell (Mouse Lewis Lung Carcinoma cells)
    Permeabilization Yes - 0.2% Triton X-100
    Gating Strategy No gating
    Specification Mouse Lewis Lung Carcinoma cells
    Preparation Cell harvesting/tissue preparation method: trypsin
    Sample buffer: Hanks’ Balanced Salt solution (HBSS)
    Fixation Paraformaldehyde
    Username

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    Verified customer

    Submitted Jan 25 2016

    Application IHC - Wholemount
    Sample Human Tissue (breast fibroadenoma)
    Specification breast fibroadenoma
    Username

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    Verified customer

    Submitted Dec 22 2015

    Application Flow Cytometry
    Sample Human Cell (Differentiated hNSCs)
    Permeabilization Yes - 0.25% Triton X-100 in DPBS
    Gating Strategy Undifferentiated Stem Cells (white)
    Specification Differentiated hNSCs
    Preparation Cell harvesting/tissue preparation method: Accutase
    Sample buffer: PBS
    Fixation Paraformaldehyde
    Username

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    Verified customer

    Submitted Sep 24 2015

    Application Western blot
    Sample African Green Monkey Cell lysate - whole cell (COS-7 cells)
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Loading amount 100000 cells
    Treatment 30 nM Bortezomib for 24 hours
    Specification COS-7 cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

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    Submitted Jul 02 2015

    Application Flow Cytometry
    Sample Human Cell (A549 lung cancer cells)
    Permeabilization Yes - 0.2% Triton X-100
    Gating Strategy No gating
    Specification A549 lung cancer cells
    Preparation Cell harvesting/tissue preparation method: trypsin
    Sample buffer: Hanks’ Balanced Salt solution (HBSS)
    Fixation Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted May 15 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (NCI-H1299 lung cancer cells)
    Specification NCI-H1299 lung cancer cells
    Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 1.5% · Temperature: 25°C
    Fixative Formaldehyde
    Username

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    Verified customer

    Submitted Mar 20 2015

    Application Western blot
    Loading amount 15 µg
    Gel Running Conditions Reduced Denaturing (4-20% Tris-glycine)
    Sample Human Cell lysate - whole cell (A549 lung cancer cells)
    Specification A549 lung cancer cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

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    Verified customer

    Submitted Feb 19 2015

    Abcam has not validated the combination of species/application used in this Abreview.
    Application ELISA
    Sample Human Recombinant protein (Human Ferritin Heavy Chain Protein (ab78877))
    Specification Human Ferritin Heavy Chain Protein (ab78877)
    Type Sandwich (Capture)
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Username

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    Verified customer

    Submitted Sep 06 2013

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