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corresponding to Human Ferritin Heavy Chain aa 150-250 (C terminal).
Database link: P02794
This product is a recombinant rabbit monoclonal antibody.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab75972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 25498860|
Unpurified ab75972 showing positive staining in normal breast tissue.
Blocking and diluting buffer: 5% NFDM/TBST
Ab75972 works better in some lysates prepared from 1% SDS Hot lysis method.
For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
ab75972 staining Ferritin Heavy Chain in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
Unpurified ab75972 at 1/100 dilution staining Ferritin Heavy Chain in human kidney tissue, using a HRP/AP polymerized secondary antibody.
Unpurified ab75972 showing positive staining in normal liver tissue.
Unpurified ab75972 showing positive staining in normal tonsil tissue.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"