Recombinant
RabMAb

Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)

Overview

  • Product name
    Anti-Ferritin Heavy Chain antibody [EPR3005Y]
    See all Ferritin Heavy Chain primary antibodies
  • Description
    Rabbit monoclonal [EPR3005Y] to Ferritin Heavy Chain
  • Specificity
    This antibody recognizes only the Ferritin heavy chain.
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Frmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    corresponding to Human Ferritin Heavy Chain aa 150-250 (C terminal).
    Database link: P02794

  • Positive control
    • WB: Human fetal liver, SH-SY5Y, human brain and HeLa cell lysates. IHC: Human kidney, breast and tonsil tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab75972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration. PubMed: 25498860
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Information by UniProt
    • Database links
    • Alternative names
      • Apoferritin antibody
      • Cell proliferation inducing gene 15 protein antibody
      • Cell proliferation-inducing gene 15 protein antibody
      • F HC antibody
      • Ferritin H subunit antibody
      • Ferritin heavy chain antibody
      • Ferritin heavy polypeptide 1 antibody
      • FHC antibody
      • FRIH antibody
      • FRIH_HUMAN antibody
      • FTH 1 antibody
      • FTH antibody
      • FTH1 antibody
      • FTH1 protein antibody
      • FTHL 6 antibody
      • FTHL6 antibody
      • Iron overload autosomal dominant antibody
      • MGC104426 antibody
      • N-terminally processed antibody
      • OK/SW-cl.84 antibody
      • PIG 15 antibody
      • PIG15 antibody
      • Placenta immunoregulatory factor antibody
      • PLIF antibody
      • Proliferation inducing gene 15 protein antibody
      • Proliferation inducing protein 15 antibody
      see all

    Images

    • Unpurified ab75972 showing positive staining in normal breast tissue.

    • All lanes : Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/1000 dilution

      Lane 1 : MCF-7 (human breast carcinoma) whole cell lysates prepared from 1% SDS Hot lysis method.
      Lane 2 : MCF-7 (human breast carcinoma) whole cell lysates prepared from RIPA lysis method
      Lane 3 : HeLa (human cervix adenocarcinoma) whole cell lysates prepared from 1% SDS Hot lysis method
      Lane 4 : HeLa (human cervix adenocarcinoma) whole cell lysates prepared from RIPA lysis method

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 21 kDa
      Observed band size : 21 kDa


      Exposure time : 3 minutes

      Blocking and diluting buffer: 5% NFDM/TBST

      Ab75972 works better in some lysates prepared from 1% SDS Hot lysis method.
      For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

       

    • ab75972 staining Ferritin Heavy Chain in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

      Negative control 1: PBS in place of primary antibody.

       

    • Lanes 1 - 3 : Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/500 dilution (unpurified)
      Lane 4 : Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/2000 dilution (unpurified)
      Lane 5 : Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/5000 dilution (unpurified)

      Lane 1 : Human liver lysate
      Lane 2 : SH-SY5Y lysate
      Lane 3 : Human brain lysate
      Lane 4 : Human brain lysate
      Lane 5 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 21 kDa
      Observed band size : 21 kDa
    • Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/5000 dilution + Human fetal liver at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 21 kDa
    • Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/2000 dilution + HeLa (human cervix adenocarcinoma) whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

      Predicted band size : 21 kDa
    • Unpurified ab75972 at 1/100 dilution staining Ferritin Heavy Chain in human kidney tissue, using a HRP/AP polymerized secondary antibody.

    • Unpurified ab75972 showing positive staining in normal liver tissue.

    • Unpurified ab75972 showing positive staining in normal tonsil tissue.

    References

    This product has been referenced in:
    • Mancone C  et al. Iron overload down-regulates the expression of the HIV-1 Rev cofactor eIF5A in infected T lymphocytes. Proteome Sci 15:18 (2017). Read more (PubMed: 28785172) »
    • Vannucci L  et al. In Vivo Targeting of Cutaneous Melanoma Using an Melanoma Stimulating Hormone-Engineered Human Protein Cage with Fluorophore and Magnetic Resonance Imaging Tracers. J Biomed Nanotechnol 11:81-92 (2015). IHC-P ; Human . Read more (PubMed: 26301302) »

    See all 7 Publications for this product

    Customer reviews and Q&As

    Application
    Flow Cytometry
    Sample
    Human Cell (Differentiated hNSCs)
    Permeabilization
    Yes - 0.25% Triton X-100 in DPBS
    Gating Strategy
    Undifferentiated Stem Cells (white)
    Specification
    Differentiated hNSCs
    Preparation
    Cell harvesting/tissue preparation method: Accutase
    Sample buffer: PBS
    Fixation
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Sep 24 2015

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    ELISA
    Sample
    Human Recombinant protein (Human Ferritin Heavy Chain Protein (ab78877))
    Specification
    Human Ferritin Heavy Chain Protein (ab78877)
    Type
    Sandwich (Capture)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 06 2013

    Vielen Dank für Ihre Anfrage.

    Zum Abreview-Verfahren: Wir möchten es unseren Kunden so einfach wie möglich machen, uns ihr Feedback mitzuteilen. Daher ist das Abreview-System als einfaches Online-Formular angelegt, das in wenig...

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    Vielen Dank für Ihre nette Anfrage.

    Da wir derzeit über 80000 Produkte in unserem Katalog verfügbar haben, können wir aus logistischen Gründen leider keine kostenlosen Proben zu Testzwecken anbieten. Hier bei Abcam ve...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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