Anti-Ferritin Light Chain antibody (ab69090)

Overview

  • Product nameAnti-Ferritin Light Chain antibody
    See all Ferritin Light Chain primary antibodies
  • Description
    Rabbit polyclonal to Ferritin Light Chain
  • Tested applicationsSuitable for: IHC-P, IHC-FoFr, IHC-Fr, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Ferritin Light Chain.

    (Peptide available as ab69089.)

  • Positive control
    • This antibody gave a positive signal in the following WB lysates: Human Liver, Mouse Spleen, Rat Testis, Mouse Liver, Mouse Ovary, Rat brain

Properties

Applications

Our Abpromise guarantee covers the use of ab69090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-FoFr Use a concentration of 1 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 21248247
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 20-21 kDa (predicted molecular weight: 19 kDa).Can be blocked with Human Ferritin Light Chain peptide (ab69089).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionStores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
  • Involvement in diseaseDefects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
    Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.
  • Sequence similaritiesBelongs to the ferritin family.
    Contains 1 ferritin-like diiron domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ferritin L chain antibody
    • Ferritin L subunit antibody
    • Ferritin light chain antibody
    • Ferritin light polypeptide antibody
    • ferritin light polypeptide like 3 antibody
    • FRIL_HUMAN antibody
    • FTL antibody
    • LFTD antibody
    • NBIA 3 antibody
    • NBIA3 antibody
    see all

Anti-Ferritin Light Chain antibody images

  • ab69090 staining Ferritin Light Chain in Rat liver tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 10% serum for 1 hour at 21°C. Samples were incubated with primary antibody (1/400 in PBS + 0.1% Triton) for 14 hours at 4°C. An Alexa Fluor® 555-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-Ferritin Light Chain antibody (ab69090) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Liver (Rat) Tissue Lysate
    Lane 3 : Mouse spleen tissue lysate - total protein (ab29293)
    Lane 4 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)
    Lane 5 : Liver (Mouse) Tissue Lysate
    Lane 6 : Ovary (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 19 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab69090 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69090, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, HepG2 and Hek293 cells at 1µg/ml, and in 4% formaldehyde fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
  • This image is of an adult mouse liver section stained with  ab69090 (perfusion fixed tissue). The animals were perfused with  4% PFA. The sections were  incubated in  5% normal donkey serum in 0.1% PBS- and triton X100  for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody (ab69090, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • All lanes : Anti-Ferritin Light Chain antibody (ab69090) at 1/500 dilution

    Lane 1 : whole brain lysate
    Lane 2 : liver treated with LPS (negative control)
    Lane 3 : liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Dako Cytomation P0260 Conjugation: HRP at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 19 kDa
    Observed band size : 20-21 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 seconds

    This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

    See Abreview

  • Immunohistochemical analysis of Human atherosclerotic coronary intima tissue, staining Ferritin Light Chain with ab69090.

References for Anti-Ferritin Light Chain antibody (ab69090)

This product has been referenced in:
  • Horniblow RD  et al. The chelation of colonic luminal iron by a unique sodium alginate for the improvement of gastrointestinal health. Mol Nutr Food Res 60:2098-108 (2016). Read more (PubMed: 27136256) »
  • Ariza J  et al. Dysregulated iron metabolism in the choroid plexus in fragile X-associated tremor/ataxia syndrome. Brain Res 1598:88-96 (2015). IHC-Fr ; Human . Read more (PubMed: 25498860) »

See all 9 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Mouse Tissue sections (heart)
Specification heart
Permeabilization No
Fixative Methanol
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Submitted Oct 01 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Mouse Tissue lysate - whole (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Oct 01 2014

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (heart)
Specification heart
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative Methanol
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Submitted Aug 18 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12)
Sample Rat Tissue lysate - whole (heart)
Specification heart
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Jul 30 2014

Application Western blot
Loading amount 40 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (15 % gel)
Sample Mouse Tissue lysate - whole (spinal cord)
Specification spinal cord
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Apr 29 2014

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Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Heart)
Specification Heart
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative Acetone
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Submitted Sep 30 2013

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Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Sample Rat Tissue sections (Liver)
Specification Liver
Permeabilization No
Fixative Acetone
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Submitted Sep 23 2013

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Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Rat Cell (hepatocytes)
Specification hepatocytes
Permeabilization No
Fixative Acetone
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Submitted Sep 20 2013

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Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Human Cell lysate - whole cell (HepG2)
Specification HepG2
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Sep 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Rat Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Sep 17 2013

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"