Overview

  • Product name
    Anti-Ferritin Light Chain antibody
    See all Ferritin Light Chain primary antibodies
  • Description
    Rabbit polyclonal to Ferritin Light Chain
  • Tested applications
    Suitable for: IHC-P, IHC-FoFr, IHC-Fr, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Ferritin Light Chain.

    (Peptide available as ab69089.)

  • Positive control
    • This antibody gave a positive signal in the following WB lysates: Human Liver, Mouse Spleen, Rat Testis, Mouse Liver, Mouse Ovary, Rat brain

Properties

Applications

Our Abpromise guarantee covers the use of ab69090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-FoFr Use a concentration of 1 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 21248247
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 20-21 kDa (predicted molecular weight: 19 kDa).Can be blocked with Human Ferritin Light Chain peptide (ab69089).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function
    Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
  • Involvement in disease
    Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
    Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.
  • Sequence similarities
    Belongs to the ferritin family.
    Contains 1 ferritin-like diiron domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ferritin L chain antibody
    • Ferritin L subunit antibody
    • Ferritin light chain antibody
    • Ferritin light polypeptide antibody
    • ferritin light polypeptide like 3 antibody
    • FRIL_HUMAN antibody
    • FTL antibody
    • LFTD antibody
    • NBIA 3 antibody
    • NBIA3 antibody
    see all

Images

  • All lanes : Anti-Ferritin Light Chain antibody (ab69090) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Liver (Rat) Tissue Lysate
    Lane 3 : Mouse spleen tissue lysate - total protein (ab29293)
    Lane 4 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)
    Lane 5 : Liver (Mouse) Tissue Lysate
    Lane 6 : Ovary (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 19 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
  • ab69090 staining Ferritin Light Chain in Rat liver tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 10% serum for 1 hour at 21°C. Samples were incubated with primary antibody (1/400 in PBS + 0.1% Triton) for 14 hours at 4°C. An Alexa Fluor® 555-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab69090 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69090, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, HepG2 and Hek293 cells at 1µg/ml, and in 4% formaldehyde fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
  • This image is of an adult mouse liver section stained with  ab69090 (perfusion fixed tissue). The animals were perfused with  4% PFA. The sections were  incubated in  5% normal donkey serum in 0.1% PBS- and triton X100  for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody (ab69090, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Immunohistochemical analysis of Human atherosclerotic coronary intima tissue, staining Ferritin Light Chain with ab69090.

References

This product has been referenced in:
  • Dietrich P  et al. Elimination of huntingtin in the adult mouse leads to progressive behavioral deficits, bilateral thalamic calcification, and altered brain iron homeostasis. PLoS Genet 13:e1006846 (2017). Read more (PubMed: 28715425) »
  • Horniblow RD  et al. The chelation of colonic luminal iron by a unique sodium alginate for the improvement of gastrointestinal health. Mol Nutr Food Res 60:2098-108 (2016). Read more (PubMed: 27136256) »

See all 17 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Rat Tissue lysate - whole (heart)
Gel Running Conditions
Reduced Denaturing (4-12)
Loading amount
30 µg
Specification
heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

Submitted May 23 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (heart)
Permeabilization
Yes - 0.3% Triton X
Specification
heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Acetone
Username

Abcam user community

Verified customer

Submitted May 09 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (heart)
Permeabilization
Yes - 0.3% Triton X
Specification
heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Acetone
Username

Abcam user community

Verified customer

Submitted May 08 2017

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample
Mouse Tissue sections (heart)
Specification
heart
Permeabilization
No
Fixative
Methanol
Username

Abcam user community

Verified customer

Submitted Oct 01 2014

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Mouse Tissue lysate - whole (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

Submitted Oct 01 2014

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (heart)
Specification
heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Methanol
Username

Abcam user community

Verified customer

Submitted Aug 18 2014

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12)
Sample
Rat Tissue lysate - whole (heart)
Specification
heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jul 30 2014

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (15 % gel)
Sample
Mouse Tissue lysate - whole (spinal cord)
Specification
spinal cord
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Apr 29 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Acetone
Username

Abcam user community

Verified customer

Submitted Sep 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Sample
Rat Tissue sections (Liver)
Specification
Liver
Permeabilization
No
Fixative
Acetone
Username

Abcam user community

Verified customer

Submitted Sep 23 2013

1-10 of 35 Abreviews or Q&A

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