Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
Involvement in disease
Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene. Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.
Belongs to the ferritin family. Contains 1 ferritin-like diiron domain.
Western blot - Anti-Ferritin Light Chain antibody (ab69090)
All lanes : Anti-Ferritin Light Chain antibody (ab69090) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889) Lane 2 : Liver (Rat) Tissue Lysate Lane 3 :Mouse spleen tissue lysate - total protein (ab29293) Lane 4 : Testis (Rat) Tissue Lysate - normal tissue (ab29388) Lane 5 : Liver (Mouse) Tissue Lysate Lane 6 : Ovary (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Immunohistochemistry (Frozen sections) - Anti-Ferritin Light Chain antibody (ab69090)This image is courtesy of an anonymous Abreview
ab69090 staining Ferritin Light Chain in Rat liver tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 10% serum for 1 hour at 21°C. Samples were incubated with primary antibody (1/400 in PBS + 0.1% Triton) for 14 hours at 4°C. An Alexa Fluor® 555-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
ICC/IF image of ab69090 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69090, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, HepG2 and Hek293 cells at 1µg/ml, and in 4% formaldehyde fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Ferritin Light Chain antibody (ab69090)This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
This image is of an adult mouse liver section stained with ab69090 (perfusion fixed tissue). The animals were perfused with 4% PFA. The sections were incubated in 5% normal donkey serum in 0.1% PBS- and triton X100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody (ab69090, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Frozen sections) - Anti-Ferritin Light Chain antibody (ab69090)Image from de la Cuesta F et al., Mol Cell Proteomics. 2011 Apr;10(4):M110.003517. doi: 10.1074/mcp.M110.003517. Epub 2011 Jan 19.Fig 5.; April 1, 2011 Molecular & Cellular Proteomics, 10, M110.003517
Immunohistochemical analysis of Human atherosclerotic coronary intima tissue, staining Ferritin Light Chain with ab69090.
Dietrich P et al. Elimination of huntingtin in the adult mouse leads to progressive behavioral deficits, bilateral thalamic calcification, and altered brain iron homeostasis. PLoS Genet13:e1006846 (2017).
Read more (PubMed: 28715425) »
Toblli JE et al. Cardiovascular, liver, and renal toxicity associated with an intravenous ferric carboxymaltose similar versus the originator compound. Drug Des Devel Ther11:3401-3412 (2017).
Read more (PubMed: 29238166) »