Anti-Ferritin Light Chain antibody (ab80585)

Overview

  • Product nameAnti-Ferritin Light Chain antibody
    See all Ferritin Light Chain primary antibodies
  • Description
    Goat polyclonal to Ferritin Light Chain
  • Tested applicationsSuitable for: Sandwich ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Horse, Cow, Cat, Dog, Pig, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Ferritin Light Chain aa 163-175 (C terminal) (Cysteine residue).
    Sequence:

    C-GEYLFERLTLKHD


    Database link: NP_000137.2

  • Positive control
    • WB: Human placenta, human cerebellum, human liver, mouse brain and rat brain tissue lysates. IHC-P: Human brain cortex tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab80585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 1 - 2 µg/ml. Used as a reporter to detect recombinant FTL
IHC-P Use a concentration of 3 - 6 µg/ml.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).

Target

Anti-Ferritin Light Chain antibody images

  • All lanes : Anti-Ferritin Light Chain antibody (ab80585) at 0.1 µg/ml

    Lane 1 : Human cerebellum tissue lysate in RIPA buffer
    Lane 2 : Human liver tissue lysate in RIPA buffer
    Lane 3 : Human placenta tissue lysate in RIPA buffer
    Lane 4 : Mouse brain tissue lysate in RIPA buffer
    Lane 5 : Rat brain tissue lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 20 kDa

    Incubated with the primary antibody for 1 hour.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain cortex tissue labelling Ferritin Light Chain with ab80585 at 3.8µg/ml. Antigen retrieval was performed with citrate buffer pH 6. HRP staining.

References for Anti-Ferritin Light Chain antibody (ab80585)

ab80585 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"