Anti-Ferritin Light Chain antibody (ab80585)

Overview

  • Product name
    Anti-Ferritin Light Chain antibody
    See all Ferritin Light Chain primary antibodies
  • Description
    Goat polyclonal to Ferritin Light Chain
  • Tested applications
    Suitable for: Sandwich ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Horse, Cow, Cat, Dog, Pig, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Ferritin Light Chain aa 163-175 (C terminal) (Cysteine residue).
    Sequence:

    C-GEYLFERLTLKHD


    Database link: NP_000137.2

  • Positive control
    • WB: Human placenta, human cerebellum, human liver, mouse brain and rat brain tissue lysates. IHC-P: Human brain cortex tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab80585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 1 - 2 µg/ml. Used as a reporter to detect recombinant FTL
IHC-P Use a concentration of 3 - 6 µg/ml.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).

Target

  • Function
    Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
  • Involvement in disease
    Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
    Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.
  • Sequence similarities
    Belongs to the ferritin family.
    Contains 1 ferritin-like diiron domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ferritin L chain antibody
    • Ferritin L subunit antibody
    • Ferritin light chain antibody
    • Ferritin light polypeptide antibody
    • ferritin light polypeptide like 3 antibody
    • FRIL_HUMAN antibody
    • FTL antibody
    • LFTD antibody
    • NBIA 3 antibody
    • NBIA3 antibody
    see all

Images

  • All lanes : Anti-Ferritin Light Chain antibody (ab80585) at 0.1 µg/ml

    Lane 1 : Human cerebellum tissue lysate in RIPA buffer
    Lane 2 : Human liver tissue lysate in RIPA buffer
    Lane 3 : Human placenta tissue lysate in RIPA buffer
    Lane 4 : Mouse brain tissue lysate in RIPA buffer
    Lane 5 : Rat brain tissue lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 20 kDa

    Incubated with the primary antibody for 1 hour.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain cortex tissue labelling Ferritin Light Chain with ab80585 at 3.8µg/ml. Antigen retrieval was performed with citrate buffer pH 6. HRP staining.

References

ab80585 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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