The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Used as a reporter to detect recombinant FTL
Use a concentration of 3 - 6 µg/ml.
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
Involvement in disease
Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene. Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.
Belongs to the ferritin family. Contains 1 ferritin-like diiron domain.
Western blot - Anti-Ferritin Light Chain antibody (ab80585)
All lanes : Anti-Ferritin Light Chain antibody (ab80585) at 0.1 µg/ml
Lane 1 : Human cerebellum tissue lysate in RIPA buffer Lane 2 : Human liver tissue lysate in RIPA buffer Lane 3 : Human placenta tissue lysate in RIPA buffer Lane 4 : Mouse brain tissue lysate in RIPA buffer Lane 5 : Rat brain tissue lysate in RIPA buffer
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain cortex tissue labelling Ferritin Light Chain with ab80585 at 3.8µg/ml. Antigen retrieval was performed with citrate buffer pH 6. HRP staining.