Recombinant
RabMAb

Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)

Overview

  • Product name
    Anti-Ferritin Light Chain antibody [EPR5260]
    See all Ferritin Light Chain primary antibodies
  • Description
    Rabbit monoclonal [EPR5260] to Ferritin Light Chain
  • Specificity
    ab109373 recognizes the light chain of Ferritin only.
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues on the C-terminus in Human Ferritin Light Chain.

  • Positive control
    • Human fetal liver, HepG2, and HeLa cell lysates, HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 19 kDa (predicted molecular weight: 20 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function
      Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
    • Involvement in disease
      Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
      Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.
    • Sequence similarities
      Belongs to the ferritin family.
      Contains 1 ferritin-like diiron domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ferritin L chain antibody
      • Ferritin L subunit antibody
      • Ferritin light chain antibody
      • Ferritin light polypeptide antibody
      • ferritin light polypeptide like 3 antibody
      • FRIL_HUMAN antibody
      • FTL antibody
      • LFTD antibody
      • NBIA 3 antibody
      • NBIA3 antibody
      see all

    Images

    • Immunohistochemical analysis of wild type and ftl knock out mouse brain tissue labeling Ferritin Light Chain. Positive staining of Ferratin Light Chain is seen in wild type mouse brain (A) and no staining is seen in the ftl knock out brain tissue (B).

    • All lanes : Anti-Ferritin Light Chain antibody [EPR5260] (ab109373) at 1/10000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 20 kDa
      Observed band size : 19 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeabilized HeLa cells using ab109373 at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:
    • Mertens C  et al. Intracellular Iron Chelation Modulates the Macrophage Iron Phenotype with Consequences on Tumor Progression. PLoS One 11:e0166164 (2016). Flow Cyt ; Human . Read more (PubMed: 27806101) »
    • Li W  et al. Systemic and cerebral iron homeostasis in ferritin knock-out mice. PLoS One 10:e0117435 (2015). WB, IHC-P ; Mouse . Read more (PubMed: 25629408) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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