The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 19 kDa (predicted molecular weight: 20 kDa).
1/10 - 1/100.
1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Application notesIs unsuitable for ICC or IHC-P.
FunctionStores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
Involvement in diseaseDefects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene. Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.
Sequence similaritiesBelongs to the ferritin family. Contains 1 ferritin-like diiron domain.
Immunohistochemical analysis of wild type and ftl knock out mouse brain tissue labeling Ferritin Light Chain. Positive staining of Ferratin Light Chain is seen in wild type mouse brain (A) and no staining is seen in the ftl knock out brain tissue (B).
Western blot - Ferritin Light Chain antibody [EPR5260] (ab109373)
All lanes : Anti-Ferritin Light Chain antibody [EPR5260] (ab109373) at 1/10000 dilution
Lane 1 : Fetal liver lysate Lane 2 : HepG2 cell lysate Lane 3 : HeLa cell lysate
Flow cytometric analysis of permeabilized HeLa cells using ab109373 at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).
References for Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
This product has been referenced in:
Mertens C et al. Intracellular Iron Chelation Modulates the Macrophage Iron Phenotype with Consequences on Tumor Progression. PLoS One11:e0166164 (2016).
Read more (PubMed: 27806101) »