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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-fetal hemoglobin antibody
See all fetal hemoglobin products (3) ...
Description
Sheep polyclonal to fetal hemoglobin
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with human hemoglobin F. No antibody was detected against hemoglobin A1, A2, S or human serum proteins.
Tested applications
ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Chimpanzee, Rhesus monkey, Gorilla
Immunogen
Human Hemoglobin F
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was isolated by affinity chromato-graphy using antigen coupled to agarose beads. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Blood >> Other
Cardiovascular >> Blood >> Serum Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab19364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
IHC-P: 1/20Antigen retrieval is not essential but may optimise staining.(PubMed: 21378008)
Target
Relevance
The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'
Cellular localization
Cytoplasmic
Alternative names
- A gamma globin antibody
- G gamma globin antibody
- Gamma 1 globin antibody
see all
Database links
- Entrez Gene: 3047 Human
- Entrez Gene: 3048 Human
- Omim: 142200 Human
- Omim: 142250 Human
- SwissProt: P69891 Human
- SwissProt: P69892 Human
- SwissProt: Q68NH9 Human
- Unigene: 302145 Human
see all
References for Anti-fetal hemoglobin antibody (ab19364)
This product has been referenced in:
- Wolk M & Martin JE Fetal haemoglobin (HbF) as an immunohistochemical tumour marker in bone marrow and spleen. J Clin Pathol : (2011). IHC-P; Human.Read more (PubMed: 21378008) »
- Zhang Xet al. In vitro maturation of erythroid progenitors from human umbilical cord blood and patterns of globin gene expression: serum from different developmental stage plays important roles in liquid culture. Biochem Biophys Res Commun 336:42-8 (2005).Read more (PubMed: 16125140) »
See all 2 publications for this product
Publishing research using ab19364? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"