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Product Name
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fetal hemoglobin antibody
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See all fetal hemoglobin antibodies (3)...
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Product type
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Primary antibodies
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Description
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Sheep polyclonal to fetal hemoglobin
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Immunogen
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Human Hemoglobin F
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Reacts with
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Hu
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Predicted to react
(species key)
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Chmp, Glla, RMk
(Due to sequence homology)
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Specificity
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By immunoelectrophoresis and ELISA this antibody reacts specifically with human hemoglobin F. No antibody was detected against hemoglobin A1, A2, S or human serum proteins.
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Tested applications
(see key)
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ELISA
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
Coating: 1:100 - 1:1000
Primary: 1:1000 - 1:30000
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Cardiovascular >> Blood >> Other
Cardiovascular >> Blood >> Serum Proteins
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Relevance
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The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Sheep
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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The antibody was isolated by affinity chromato-graphy using antigen coupled to agarose beads. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this fetal hemoglobin antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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References for fetal hemoglobin antibody (ab19364)
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This product has been used in:
Zhang X et al. In vitro maturation of erythroid progenitors from human umbilical cord blood and patterns of globin gene expression: serum from different developmental stage plays important roles in liquid culture. Biochem Biophys Res Commun 336:42-8 (2005). PubMed: 16125140
If you publish research using ab19364 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to fetal hemoglobin
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fetal hemoglobin antibody - more information
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Customer reviews (feedback) regarding fetal hemoglobin antibody |
Customer FAQs regarding fetal hemoglobin antibody |
Protocols for fetal hemoglobin antibody |
Price and availability of products related to fetal hemoglobin antibody |
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fetal hemoglobin antibody - related products:
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fetal hemoglobin antibody - other tools:
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Contact Abcam with a Technical Enquiry about fetal hemoglobin antibody |
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