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Product Name 

fetal hemoglobin antibody

 

See all fetal hemoglobin antibodies (3)...

Product type 

Primary antibodies

Description 

Sheep polyclonal to fetal hemoglobin

Immunogen 

Human Hemoglobin F

Reacts with 

Hu

Predicted to react 
(species key)

Chmp, Glla, RMk
(Due to sequence homology)

Specificity 

By immunoelectrophoresis and ELISA this antibody reacts specifically with human hemoglobin F. No antibody was detected against hemoglobin A1, A2, S or human serum proteins.

Tested applications 
(see key)

ELISA


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
Coating: 1:100 - 1:1000
Primary: 1:1000 - 1:30000

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Cardiovascular >> Blood >> Other
Cardiovascular >> Blood >> Serum Proteins

Relevance 

The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'

 

Alternative names fetal hemoglobin antibody (ab19364)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

3047    (Human)
3048    (Human)

Omim

    

142200    (Human)
142250    (Human)

SwissProt

    

P69892    (Human)
P69891    (Human)
Q68NH9    (Human)

Unigene

    

530049    (Human)
302145    (Human)

Raised in 

Sheep

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

The antibody was isolated by affinity chromato-graphy using antigen coupled to agarose beads. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this fetal hemoglobin antibody is on this datasheet. But please do contact us if you would like any reassurance!



References for fetal hemoglobin antibody (ab19364)

Specific publication references   This product has been used in:

Zhang X et al. In vitro maturation of erythroid progenitors from human umbilical cord blood and patterns of globin gene expression: serum from different developmental stage plays important roles in liquid culture. Biochem Biophys Res Commun 336:42-8 (2005). PubMed: 16125140

If you publish research using ab19364 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to fetal hemoglobin

 

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fetal hemoglobin antibody - related products:

 

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ab7009

  

Phycoerythrin antibody

 

Other fetal hemoglobin Antibodies

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