Recombinant
RabMAb

Anti-fetal hemoglobin antibody [EPR9709] (ab156584)

Overview

  • Product name
    Anti-fetal hemoglobin antibody [EPR9709]
    See all fetal hemoglobin primary antibodies
  • Description
    Rabbit monoclonal [EPR9709] to fetal hemoglobin
  • Specificity
    ab156584 recognises Hemoglobin gamma-1/2 chain.
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human fetal hemoglobin

  • Positive control
    • Red blood cells and K562 lysates, K562 cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR9709
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab156584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 15 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Relevance
      The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'
    • Cellular localization
      Cytoplasmic
    • Database links
    • Alternative names
      • A gamma globin antibody
      • G gamma globin antibody
      • Gamma 1 globin antibody
      • Gamma 2 globin antibody
      • Gamma A hemoglobin antibody
      • Gamma globin antibody
      • Hb F Agamma antibody
      • Hb F Ggamma antibody
      • hbfagamma antibody
      • hbfggamma antibody
      • HBG 1 antibody
      • HBG 2 antibody
      • HBG1 antibody
      • HBG2 antibody
      • HBGA antibody
      • HBGR antibody
      • Hemoglobin gamma 1 chain antibody
      • Hemoglobin gamma 2 chain antibody
      • Hemoglobin gamma A antibody
      • Hemoglobin gamma A chain antibody
      • Hemoglobin gamma G antibody
      • Hemoglobin gamma G chain antibody
      • Hemoglobin gamma regulator of antibody
      • Hemoglobin subunit gamma 1 antibody
      • Hemoglobin subunit gamma 2 antibody
      • HSGGL 1 antibody
      • HSGGL1 antibody
      • Methemoglobin antibody
      • PRO 2979 antibody
      • PRO2979 antibody
      see all

    Images

    • All lanes : Anti-fetal hemoglobin antibody [EPR9709] (ab156584) at 1/2000 dilution

      Lane 1 : Human red blood cell
      Lane 2 : K562(human lymphoblast from chronic myelogenous leukemia) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) at 1/1000 dilution

      Predicted band size : 15 kDa

      Blocking and Diluting buffer:5% NFDM/TBST

      Exposure time:3 min

    References

    This product has been referenced in:
    • Pule GD  et al. Hydroxyurea down-regulates BCL11A, KLF-1 and MYB through miRNA-mediated actions to induce ?-globin expression: implications for new therapeutic approaches of sickle cell disease. Clin Transl Med 5:15 (2016). WB ; Human . Read more (PubMed: 27056246) »

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab156584.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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