Overview

  • Product nameFetuin A (AHSG) Human ELISA Kit
    See all Alpha 2 HS Glycoprotein kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5.1%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.1%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    > 3 ng/ml
  • Range
    3.13 ng/ml - 200 ng/ml
  • Recovery

    = 99 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Fetuin A (AHSG) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fetuin A concentrations in Human plasma, serum, urine, milk and cell culture supernatants.

    A Fetuin A specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fetuin A specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fetuin A captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Fetuin A (AHSG) Human ELISA Kit images

  • Representative Standard Curve using ab108855.

Protocols

References for Fetuin A (AHSG) Human ELISA Kit (ab108855)

This product has been referenced in:
  • Májek P  et al. Plasma proteome changes associated with refractory anemia and refractory anemia with ringed sideroblasts in patients with myelodysplastic syndrome. Proteome Sci 11:14 (2013). Read more (PubMed: 23566303) »
  • Kuakarn S  et al. Serum proteins in chronic hepatitis B patients treated with peginterferon alfa-2b. World J Gastroenterol 19:5067-75 (2013). Sandwich ELISA ; Human . Read more (PubMed: 23964140) »

See all 2 Publications for this product

Product Wall

Thank you for your patience, I apologize for the delay in my reply. The lab has confirmed that this kit will not cross-react with bovine alpha 2 HS Glycoprotein, and that FBS will not interfere with your results. I hope this helps, please let me know i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"