Overview

  • Product nameFetuin A (AHSG) Human ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    312 pg/ml - 20000 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human Fetuin A (AHSG) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Fetuin A (AHSG) in cell culture supernatants, serum and plasma (heparin, EDTA).

    A Fetuin A (AHSG) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Fetuin A (AHSG) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Fetuin A (AHSG) amount of sample captured in plate.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionPromotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.
  • Tissue specificitySynthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins.
  • Sequence similaritiesBelongs to the fetuin family.
    Contains 2 cystatin fetuin-A-type domains.
  • Post-translational
    modifications
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession P02765 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • A2HS
    • AHS
    • Ahsg
    • Alpha 2 HS glycoprotein
    • Alpha-2-HS-glycoprotein chain B
    • Alpha-2-Z-globulin
    • Ba-alpha-2-glycoprotein
    • FETUA
    • FETUA_HUMAN
    • Fetuin-A
    • HSGA
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

Fetuin A (AHSG) Human ELISA Kit images

  • Representative Standard Curve using ab119594

Protocols

References for Fetuin A (AHSG) Human ELISA Kit (ab119594)

ab119594 has not yet been referenced specifically in any publications.

Product Wall

ab119594 has not been tested with mouse samples, and we do not recommend it for use with mouse samples. We also do not recommend it's use with rat samples either.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"