Overview

  • Product name
  • Description
    Goat polyclonal to Fez1
  • Specificity
    ab53562 is expected to recognize isoform 1 (NP_005094.1) only.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Cynomolgus monkey
  • Immunogen

    Synthetic peptide:

    C-QNKQKEQRELMKK

    , corresponding to internal sequence amino acids 278-290 of Human Fez1

  • Positive control
    • Human Brain (frontal Cortex) lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab53562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

Target

  • Function
    May be involved in axonal outgrowth as component of the network of molecules that regulate cellular morphology and axon guidance machinery. Able to restore partial locomotion and axonal fasciculation to C.elegans unc-76 mutants in germline transformation experiments. May participate in the transport of mitochondria and other cargos along microtubules.
  • Tissue specificity
    Mainly expressed in brain.
  • Sequence similarities
    Belongs to the zygin family.
  • Post-translational
    modifications
    Phosphorylated by protein kinase C zeta; which enhances interaction with UBE4B and polyubiquitination.
    Polyubiquitinated in a UBE4B-dependent manner; which does not lead to proteasomal degradation and may be important for neurogenic activity. Polyubiquitin linkage seems to be mainly through Lys-26.
  • Cellular localization
    Cytoplasm > cytoskeleton > centrosome. Cell membrane. Co-localizes with both, alpha- and gamma-tubulin. Translocated from the plasma membrane to the cytoplasm by activation of the PKC zeta.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fasciculation and elongation protein zeta 1 antibody
    • Fasciculation and elongation protein zeta-1 antibody
    • Fez 1 antibody
    • Fez antibody
    • FEZ1 antibody
    • FEZ1_HUMAN antibody
    • Fezl antibody
    • Zygin 1 antibody
    • Zygin I antibody
    • Zygin-1 antibody
    • Zygin1 antibody
    • ZyginI antibody
    see all

Anti-Fez1 antibody images

  • Anti-Fez1 antibody (ab53562) at 0.03 µg/ml + Human Brain (frontal Cortex) lysate (in RIPA buffer) at 35 µg

    Predicted band size : 45 kDa
    Observed band size : 45 kDa

References for Anti-Fez1 antibody (ab53562)

This product has been referenced in:
  • McKnight NC  et al. Genome-wide siRNA screen reveals amino acid starvation-induced autophagy requires SCOC and WAC. EMBO J : (2012). WB ; Human . Read more (PubMed: 22354037) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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